I am thrilled to share the first manuscript from the Wiles lab! We present "Phollow", an in vivo phage-tagging approach that enables direct observation of phage outbreaks with single-virion resolution by live imaging. Here some highlights 👇 www.nature.com/articles/s41... - ThreadSky

ortizdeora.bsky.social • 28 days ago

I am thrilled to share the first manuscript from the Wiles lab! We present "Phollow", an in vivo phage-tagging approach that enables direct observation of phage outbreaks with single-virion resolution by live imaging. Here some highlights 👇 https://www.nature.com/articles/s41564-025-01981-1#Abs1

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liliancaesar.bsky.social•26 days ago

Congrats, Liz! Beautiful work!!!

ortizdeora.bsky.social•26 days ago

Thank you. So nice to hear from you!

markjmandel.bsky.social•27 days ago

Beautiful, clever work. Congratulations!

mulveylab.bsky.social•23 days ago

Congrats! Beautiful work!

ortizdeora.bsky.social•23 days ago

Thank you!

microbesinfo.bsky.social•27 days ago

👍

ortizdeora.bsky.social•28 days ago

One of my favorite experiments with Phollow are time lapses that allow us to illuminate the secret lives of phages. Here, we captured P2 temperate phages undergoing lytic replication in E. coli, leading to explosive host cell lysis and rapid virion dispersal into the environment.

ortizdeora.bsky.social•28 days ago

Phollow enables tracking chains of interbacterial transmission. As phages infect new bacterial hosts, they’re re-tagged with a new fluorescent color. Here, green virions from green-labeled cells infect pink- and cyan-labeled targets, generating vibrant confetti bursts of differently colored phages.

ortizdeora.bsky.social•28 days ago

We then brought Phollow into the gut of live zebrafish, taking advantage of their transparency and small size to visualize microbial communities across the entire intestine —capturing their dynamic interactions with the host in real time! pink = DNA, purple = actin, green = E. coli

ortizdeora.bsky.social•28 days ago

The following videos reveals the aftermath of a phage outbreak: virions dispersed throughout the lumen...

ortizdeora.bsky.social•28 days ago

virions closely associating with the intestinal mucosa and tissue...

gutmichaelbio.me•26 days ago

@corbin13isodope.bsky.social this is so cool!

ortizdeora.bsky.social•28 days ago

phages (in white) being internalized by gut-lining cells, including enteroendocrine cells (in green). pink = nuclei of intestinal tissues.

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