Realfreq, a framework for real-time base modification analysis for nanopore sequencing, is now published in Bioinformatics.
Written efficiently using C, a laptop can keep up with a
@nanoporetech.com MinION sequencer, and a desktop a PromethION 2 solo flowcell.
https://academic.oup.com/bioinformatics/article/41/4/btaf151/8107218