Our paper on 96-well control of temperature is now out @cp-cellsystems.bsky.social. Exceedingly proud of this work led by Will Benman, plus Pavan Iyengar, @trmumford.bsky.social, and much of our lab. Come for DIY hardware, stay for the stress granule dynamics and memory🧵 📝 tinyurl.com/thermoPlate - ThreadSky

bugajlab.bsky.social • 93 days ago

Our paper on 96-well control of temperature is now out @cp-cellsystems.bsky.social. Exceedingly proud of this work led by Will Benman, plus Pavan Iyengar, @trmumford.bsky.social, and much of our lab. Come for DIY hardware, stay for the stress granule dynamics and memory🧵

📝 https://tinyurl.com/thermoPlate

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bugajlab.bsky.social•93 days ago

The thermoPlate addresses the lack of methods for fast/accurate/precise/robust control of temperature for mammalian cells, in high throughput, under a microscope. Like the optoPlate but for temperature, including 96x PID controllers for precision.

bugajlab.bsky.social•93 days ago

Annoyingly, thermodynamics says heat will not stay confined to individual wells, constraining the complexity of our experiments. But we can predict heat spread dynamics over time with our online app, letting us quickly know if our desired experiment is possible.
https://bugajlab.shinyapps.io/ThermoPlate_GUI/

bugajlab.bsky.social•93 days ago

We used the thermoPlate to explore stress adaptation to heat shock.We found beautiful stress granule dynamics and memory formation, with differences at each degree of heating. What started as a quick peek at stress granules is now an exciting new avenue of inquiry for the lab.

bugajlab.bsky.social•93 days ago

Many additional details and surprises in the paper. Let us know if you are interested in the thermoPlate. Our goal was for others to build and use in their own labs. Big thanks to NSF, NIH, the US taxpayer, and Penn CPE4H for funding. And @cp-cellsystems.bsky.social for the excellent pub process💯.

bugajlab.bsky.social•93 days ago

Hmm -- issues with the GIFs above: here is the first!
https://www.sciencedirect.com/science/article/pii/S2405471225000675

bugajlab.bsky.social•93 days ago

and the second
https://www.sciencedirect.com/science/article/pii/S2405471225000675

sunnyshinlab.bsky.social•93 days ago

Congratulations Lukasz and the rest of the authors on this exciting new tool and finding!

bugajlab.bsky.social•93 days ago

Thank you Sunny!

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