Can AI-designed binders degrade, aggregate, stain and enrich endogenous proteins in human cells? Check out our new preprint! www.biorxiv.org/content/10.1... - ThreadSky

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Can AI-designed binders degrade, aggregate, stain and enrich endogenous proteins in human cells? Check out our new preprint!
https://www.biorxiv.org/content/10.1101/2025.05.11.653342v1

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jsb-lab.bsky.social•17 days ago

We developed a technology called Transposon-Display, which couples AI binders to their own DNA for pooled screening

jsb-lab.bsky.social•17 days ago

Using Transposon-Display, we identified binders for four intracellular human proteins and further improved them by saturation mutagenesis

jsb-lab.bsky.social•17 days ago

Coupling AI-binders to EGFP enabled the antibody-free staining of an endogenous protein in primary human macrophages

jsb-lab.bsky.social•17 days ago

Fusing AI-binders to a mutant FKBP12 domain enabled drug-controlled degradation of the endogenous target protein in human macrophages

jsb-lab.bsky.social•17 days ago

Not yet in the preprint: Mass spectrometry confirmed the highly specific degradation of one target protein out of 9,677 human proteins that were quantified in cell lysates

jsb-lab.bsky.social•17 days ago

This work was led by the phenomenal team of Alexander Hoch, Caroline Fandrey, Thomas Ebert in collaboration with Brian Coventry and David Baker at @uwproteindesign.bsky.social, Tim Kempchen at the Hölzel lab, and Sebastian Virreira Winter and Philipp Geyer from https://ions.bio

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