But then I threw a wrench into the whole story by saying "let's do the in vitro biochemistry" 🤡 our lab is a not a biochemistry lab so I had to learn everything from the ground up 🙃 (there were some days of lying on the ground and contemplating existence) - ThreadSky

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springcyclone.bsky.social • 20 days ago

But then I threw a wrench into the whole story by saying "let's do the in vitro biochemistry" 🤡 our lab is a not a biochemistry lab so I had to learn everything from the ground up 🙃

(there were some days of lying on the ground and contemplating existence)

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springcyclone.bsky.social•20 days ago

The in vitro biochemistry demonstrates that DPY-27 can hydrolyze ATP and that the EQ mutation abolishes this function

Our mutant worms demonstrate that losing this ATPase function is equally severe to losing the functional protein (EQ vs indel worms).

springcyclone.bsky.social•20 days ago

We also found that loss of this function can prevent condensin I-DC from strongly binding DNA but it is random, leaving 2 populations – strongly DNA bound and weakly DNA bound, contributing to the lack of dosage compensation and maternal effect lethality in offspring

springcyclone.bsky.social•20 days ago

Dosage compensation in worms is a paradigm for chromosome-wide gene regulation by a condensin as C. elegans don't completely shut down one X like mammals. Instead, each X is downregulated to have 1/2 expression by condensin I-DC and together both X's = single male X.

springcyclone.bsky.social•20 days ago

By demonstrating that condensin I-DC has an active ATPase that's required for dosage compensation, we hopefully apply these findings to other scenarios where condensins mediate gene regulation

#condensin #generegulation #chromatin #celegans 🪱

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