A Christmas present to all of the lasso peptide connoisseurs out there. The next chapter in our work is on triculamin is ready on bioRxiv. Great work by two incredibly talented graduate students @amerrild.bsky.social and @tizianasvenningsen.bsky.social. www.biorxiv.org/content/10.1... - ThreadSky

About ThreadSky

thomastorring.bsky.social • 71 days ago

A Christmas present to all of the lasso peptide connoisseurs out there. The next chapter in our work is on triculamin is ready on bioRxiv. Great work by two incredibly talented graduate students @amerrild.bsky.social and @tizianasvenningsen.bsky.social.
https://www.biorxiv.org/content/10.1101/2024.12.20.629632v1

Comments

thomastorring.bsky.social•71 days ago

Two years ago, we reported that triculamin and alboverticillin - two identical compounds isolated in the 1950-60's were in fact lasso peptides. Back then, we were puzzled by the biosynthesis, that didn't appear to follow the canonical lasso peptide logic. No leader peptide, no RRE, and no peptidase?

thomastorring.bsky.social•71 days ago

Now we show through heterologous expression in S. albus, that the biosynthesis is non-canonical and appear to need only a precursor peptide with a follower and a macrocyclase. And an acetyltransferase as a self-resistance gene.

thomastorring.bsky.social•71 days ago

But even more surprisingly, we find normal canonical BGC that leads to very similar triculamin-like lasso peptides across Pseudomonadota and Bacilliota. We characterize one such lasso peptide from C. palmae produced in Burkholderia, and one from B. gelatini.

thomastorring.bsky.social•71 days ago

The lasso peptide, gelatinamin, from B. gelatini contains an unusual additional macrocyclization similar to lariocidin recently reported by Wright et al. (https://doi.org/10.21203/rs.3.rs-5058118/v1)

thomastorring.bsky.social•71 days ago

Through a great collaboration with @wildtypemc.bsky.social, we take a closer look at the macrocyclases across all lasso peptide BGC, and in particular, the split between canonical and non-canonical BGCs. A final thanks to Carlsberg Fonden for the funding.

Posting Rules

Be respectful to others
No spam or self-promotion
Stay on topic
Follow Bluesky's terms of service

Comments

Posting Rules

Reply