Excited to share our new preprint on mutational scanning of the TnpB RNP! We identify several highly activating, single-position mutations in TnpB’s protein and reRNA. These datasets enabled us to engineer enhanced TnpB protein variants (>50x plant editing levels compared to WT).
Reposted from
Savage Lab
What's better than 1 deep mutational scanning (DMS) library? 2! In a new pre-print @brittneywthornton.bsky.social and @rfw.bsky.social et al. map the mutational landscape of ISDra2 TnpB protein and reRNA and leverage these datasets to engineer highly active variants (1/9)
Comments
@rfw.bsky.social and I hope our characterization of several evolutionarily-accessible, activating mutations will provide insights into TnpB evolution and TnpB’s function within insertion sequences. Congrats to the entire amazing team!