1/ One more preprint before the holidays! In which we find that chromatin compaction scales with cell size from interphase through mitosis. A collaboration from waaay back (see end of thread) with Melike Lakadamyali @melikel.bsky.social and Jerome Solon. A thread: 👇 www.biorxiv.org/content/10.1... - ThreadSky

longchrom.bsky.social • 79 days ago

1/ One more preprint before the holidays! In which we find that chromatin compaction scales with cell size from interphase through mitosis. A collaboration from waaay back (see end of thread) with Melike Lakadamyali @melikel.bsky.social and Jerome Solon. A thread: 👇
https://www.biorxiv.org/content/10.1101/2024.12.05.627016v1

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longchrom.bsky.social•79 days ago

2/ As cells divide during development, their size evolves while DNA content remains constant. For instance, Drosophila large neural stem cells (neuroblasts) generate smaller ganglion mother cells (GMCs). How does chromosome compaction adjust to these size changes?

longchrom.bsky.social•79 days ago

3/ Previous studies in worms and frogs showed that chromosome compaction in mitosis is somehow proportional to the size of the nucleus in interphase. But how does nuclear volume influence interphase DNA density? Conventional microscopy struggles to resolve this due to the dense packing of chromatin.

longchrom.bsky.social•79 days ago

4/ To address this, we used super-resolution STORM microscopy to generate detailed spatial maps of DNA organisation in interphase nuclei. Using 3D super-resolution STORM imaging, we found that nanoscale chromatin density follows a power law relationship with nuclear volume.

longchrom.bsky.social•79 days ago

5/ This scaling reflects molecular regulation rather than mere physical packing—when we blocked histone deacetylase activity, the precise relationship broke down.

longchrom.bsky.social•79 days ago

6/ To extend this finding to mitosis, we developed a novel method to quantify chromatin volume using fluorescence intensity distributions, bypassing limitations of traditional segmentation. This allowed us to track compaction dynamics live.

longchrom.bsky.social•79 days ago

7/ We found that power law scaling persists into mitosis, though the scaling exponent decreases. This reflects a shift in chromatin behavior during mitotic compaction, likely linked to phase separation dynamics, that explains how the memory from interphase compaction is carried over to mitosis.

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