🚨 Our #singlecell article is out! We describe the Chip-Tip label-free SCP workflow quantifying >5000 proteins in single HeLa cells. With high scalability, increased depth and throughput we can now envision large-scale LFQ-SCP biomedical studies! www.nature.com/articles/s41... - ThreadSky

About ThreadSky

jesperolsenlab.bsky.social • 42 days ago

🚨 Our #singlecell article is out! We describe the Chip-Tip label-free SCP workflow quantifying >5000 proteins in single HeLa cells. With high scalability, increased depth and throughput we can now envision large-scale LFQ-SCP biomedical studies!
https://www.nature.com/articles/s41592-024-02558-2

Comments

proteomicsnews.bsky.social•42 days ago

Fantastic study! Why the silly name?

jesperolsenlab.bsky.social•42 days ago

Throughput is crucial in LFQ-SCP! We tested the recently introduced Whisper Zoom 80 and 120 SPD providing impressive proteome coverage at up to 3x increased throughput.

jesperolsenlab.bsky.social•42 days ago

Using the FAIMS Pro Duo coupled to the Vanquish Neo, we even reached >6000 proteins quantified in single HeLa cells at 80 SPD throughput.

jesperolsenlab.bsky.social•42 days ago

We benchmarked the SCP workflow using Spectronaut and DIA-NN including estimation of the error rate of identifications using a shuffled human mimic protein database.

jesperolsenlab.bsky.social•42 days ago

The improved sensitivity and peptide coverage allows for the detection of abundance PTM sites harboring phosphorylations and glycosylation without prior enrichment, opening new opportunities for SCP analysis.

jesperolsenlab.bsky.social•42 days ago

We further investigated the effect of 5-FU treatments on spheroids with the whisper 80SPD gradient, highlighting differential regulation of some 5-FU targets.

jesperolsenlab.bsky.social•42 days ago

Lastly we applied the workflow to analyse undirected stem cell differentiation enabling the accurate quantification of stem cells and lineage markers.

Posting Rules

Be respectful to others
No spam or self-promotion
Stay on topic
Follow Bluesky's terms of service

Comments

Posting Rules

Reply