Thus, we have focused in engineering C. acnes. Now, we offer a whole set of tools for making C. acnes smarter with: 🔨 Modular and optimized plasmid 🔨 Basic expression tools: promoters, RBSs, terminators… 🔨 CRISPRi 🔨 Natural and synthetic inducible systems - ThreadSky

gnevot.xyz • 17 days ago

Thus, we have focused in engineering C. acnes. Now, we offer a whole set of tools for making C. acnes smarter with:
🔨 Modular and optimized plasmid
🔨 Basic expression tools: promoters, RBSs, terminators…
🔨 CRISPRi
🔨 Natural and synthetic inducible systems

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gnevot.xyz•17 days ago

How did we apply these tools? We focused mainly on two objectives:
1️⃣ Biocontainment strategies for safely deploying C. acnes in human skin 🛑🦠
2️⃣ A proof-of-concept therapy to combat UV-induced oxidative damage ☀️💥

gnevot.xyz•17 days ago

For biocontainment, we focused on targeting key metabolic genes so C. acnes would only survive if we topically supplement the missing amino acid. Since knocking out genes is still challenging in this skin non-model bacterium, we turned to #CRISPRi to quickly screen auxotrophs.

gnevot.xyz•17 days ago

For therapeutics, we implemented a UV-damage kreatinocyte model and see if the supernatant of an engineered C. acnes producing a recombinant antioxidant protein could protect the cells. Turns out it reduced significantly the ROS levels in the UV exposed keratinocytes!

gnevot.xyz•17 days ago

Very nice Guille, so what’s next? We hope to have demonstrated the potential of C. acnes to become the best microbial chassis for synbio applications in the skin and we are working to validate this in more realistic models so stray tuned!

gnevot.xyz•17 days ago

Of course, this work would not have been possible without the funding from the @ec.europa.eu #EIT #SKINDEV, the mentorship of @marcguellc.bsky.social and the efforts from the team at #SynbioLabUPF @upf.edu @prbb.org with special thanks to #JavierSantos!)...

gnevot.xyz•17 days ago

Also thanks to our great skindev collaborators #EllenBogaard #PatrickJansen from @radboudumc.bsky.social and #IçvaraBarbier #RodrigoLedesma from @imperialcollegeldn.bsky.social
. Also, thanks to our great editor #ErnestoAndrianantoandro for all his enthusiasm and guidance over the publication.

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