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jcornlab.bsky.social
•
45 days ago
We call this "RaPID-seq". It unveils a new dimension of DNA repair, telling us what is searched during HDR. In other words, what are the candidates?
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jcornlab.bsky.social
•
45 days ago
Search is constrained by chromatin conformation. Check out the related preprints from
https://fedeteloni.bsky.social
and
https://albertomarin.bsky.social
for a much deeper dive (see later in thread).
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jcornlab.bsky.social
•
45 days ago
Only 3D-nearby those sequences that are searched (i.e., candidates) are chosen as HDR donors! Donors too far away are never seen and never used.
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jcornlab.bsky.social
•
45 days ago
During genome editing, exogenous donor DNAs soak up homology search during HDR. Even if they have no sequence relationship to the cut site at all. They actually steal search from the genome!
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jcornlab.bsky.social
•
45 days ago
Altogether, proximity is key and search normally stays around the DSB. When it comes choosing a donor, only these sequences are candidates.
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jcornlab.bsky.social
•
45 days ago
This project was driven by amazing graduate student
https://charlesyeh.bsky.social
, with lots of amazing collaborators over the years.
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[–]
jcornlab.bsky.social
•
45 days ago
Curious about how cohesin guides homology search via loops & sister chromatid linkages? Check out this new preprint by
https://fedeteloni.bsky.social
&
https://gerlichlab.bsky.social
! 👉
https://tinyurl.com/DSBcohesin
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and https://albertomarin.bsky.social for a much deeper dive (see later in thread).