Search is constrained by chromatin conformation. Check out the related preprints from fedeteloni.bsky.social and albertomarin.bsky.social for a much deeper dive (see later in thread). - ThreadSky

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jcornlab.bsky.social • 45 days ago

Search is constrained by chromatin conformation. Check out the related preprints from https://fedeteloni.bsky.social
and https://albertomarin.bsky.social for a much deeper dive (see later in thread).

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jcornlab.bsky.social•45 days ago

Only 3D-nearby those sequences that are searched (i.e., candidates) are chosen as HDR donors! Donors too far away are never seen and never used.

jcornlab.bsky.social•45 days ago

During genome editing, exogenous donor DNAs soak up homology search during HDR. Even if they have no sequence relationship to the cut site at all. They actually steal search from the genome!

jcornlab.bsky.social•45 days ago

Altogether, proximity is key and search normally stays around the DSB. When it comes choosing a donor, only these sequences are candidates.

jcornlab.bsky.social•45 days ago

This project was driven by amazing graduate student https://charlesyeh.bsky.social, with lots of amazing collaborators over the years.

jcornlab.bsky.social•45 days ago

Curious about how cohesin guides homology search via loops & sister chromatid linkages? Check out this new preprint by https://fedeteloni.bsky.social & https://gerlichlab.bsky.social! 👉 https://tinyurl.com/DSBcohesin

jcornlab.bsky.social•45 days ago

If you’re interested in how loop-extruding cohesin drives the homology search during HR, check out also this new preprint from https://albertomarin.bsky.social in the Taekjip Ha lab (https://doi.org/10.1101/2025.02.10.637451)

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