Using the peace and silence of an early Saturday morning to run a few sample 🫠 #Phdlife #labrat #peroxisomes #proteomics #IchBinHanna and #TeamMassSpec - ThreadSky

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dwendscheck.bsky.social • 40 days ago

Using the peace and silence of an early Saturday morning to run a few sample 🫠
#Phdlife #labrat #peroxisomes #proteomics #IchBinHanna and #TeamMassSpec

Comments

pommie331.bsky.social•39 days ago

Self poured or pre-cast gels? I remember those days back in the 80's.

dwendscheck.bsky.social•39 days ago

Self poured in empty ready-to-use gel cassettes. Makes a hell of a plastic waste but is my PIs compromise between self-assembled glass cassettes and pre-casted gels. With several MS-instruments next door i also feel like working in the stone-age when doing Western blots 🙃😅

pommie331.bsky.social•39 days ago

Lots of respect from me. Too many students and post docs use pre cast without ever knowing the ins and outs of SDS PAGE. Costs plenty too!!

msbioworks.bsky.social•40 days ago

Also, why are you Hanna?

dwendscheck.bsky.social•40 days ago

https://de.m.wikipedia.org/wiki/IchBinHanna
Sadly just in German Wikipedia available. It's a hashtag initiated by @amreibahr.bsky.social and others to raise attention for working conditions of Postdocs and Phds in 🇩🇪

msbioworks.bsky.social•40 days ago

What's the experiment?

dwendscheck.bsky.social•40 days ago

Subcellular fractionation of yeast cells with impaired peroxisomal function. Western blotting to test samples before generating MS data 🫡

msbioworks.bsky.social•40 days ago

Cool. What technique do you use for fractionation?

dwendscheck.bsky.social•40 days ago

Simple differential centrifugation.

msbioworks.bsky.social•40 days ago

Cool. Good luck with your Western's.

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