1/n Wanted to share this latest piece of work that came online today on how Scale-independent and Topological interactions govern the spindle orientation and in turn the decision to become embryonic/yolk nuclei. Freely available to read here: rdcu.be/ebb0l - ThreadSky

argo-mkhrji.bsky.social • 32 days ago

1/n Wanted to share this latest piece of work that came online today on how Scale-independent and Topological interactions govern the spindle orientation and in turn the decision to become embryonic/yolk nuclei.

Freely available to read here:
https://rdcu.be/ebb0l

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argo-mkhrji.bsky.social•32 days ago

2/n It was very exciting theory-driven analysis and cutting age imaging experiment ( @woonyunghur.bsky.social during his time in the lab of @ditalialab.bsky.social ) collaboration across the atlantic between @qbio-ens.bsky.social (M.Vergassola)and @dukepress.bsky.social

argo-mkhrji.bsky.social•32 days ago

3/n As a physicist, how in the shared cytoplasm of a syncytial system biochemical identity of nuclei emerge has been always puzzling to me, starting even during my PhD @mpicbg.bsky.social (on C. elegans germline). Generically true for all germlines, slime mold, ashbya etc. Also for fly embryo

argo-mkhrji.bsky.social•32 days ago

4/n majority of the fly embryo nuclei must travel from the center of the large embryo to the cortex to give rise to the future embryonic layer but some must stay behind to give rise to the yolk nuclei. @woonyunghur.bsky.social captured this with @npmitchell.bsky.social & @streichan.bsky.social

argo-mkhrji.bsky.social•32 days ago

5/n In many ways this is the first fate decision at the fly embryo. Ziqi Lu looked into it by reanalysing some published data the MZT in the fly.

argo-mkhrji.bsky.social•32 days ago

6/n So what determines which nuclei end up at the cortex of the egg giving rise to the beautiful little fly embryo that we know and love and which ones stay behind to make the yolk nuclei ?

The answer lies in the orientation of nuclear division (the primary driver of motion at this stage).

argo-mkhrji.bsky.social•32 days ago

7/n Like many others we thought (mostly M. Vergassola), perhaps nuclear packing and pushing forces between different spindles (later important during cycle 10 onwards) could give rise to some sort of density dependent flip instability. But to @woonyunghur.bsky.social's and my surprise: No.

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