We used a Single Objective Light-Sheet (#Snouty, for the friends).
It is less invasive than a lattice light sheet because it requires a smaller dissection area on the head´s cuticula, as it is only necessary to accommodate the light cone of a single objective. And it is simpler. 2/6
It is less invasive than a lattice light sheet because it requires a smaller dissection area on the head´s cuticula, as it is only necessary to accommodate the light cone of a single objective. And it is simpler. 2/6
Comments
The NA=1.1 allowed high spatial resolution.
Here, a line intensity profile showing features as small as 360 nm.
3/6
We can image the fly for 30 minutes without bleaching it, and can get 10 times more images than with confocal microscopy!
Also, we don't rely on phototoxic pulsed infrared lasers like in 2-photon microscopy.
4/6
Here the membrane is labelled in green (GFP), and dense core vesicles are labeled in magenta (mCherry).
We also show mitochondria imaging in the manuscript.
5/6
Big thanks also to @amsikking.bsky.social , @andrewgyork.bsky.social and
@lumasullo.bsky.social, they have always been there to help.
Stay tuned for new imaging on living Drosophila!
6/6