Gene expression analysis in metabolic tissues revealed some interesting changes in transcriptional programs related to futile cycles (body using energy somehow like the guy in the GIF below), but the impact of these changes in the response to overfeeding remains to be determined. (5/n) - ThreadSky

pranea.bsky.social • 44 days ago

Gene expression analysis in metabolic tissues revealed some interesting changes in transcriptional programs related to futile cycles (body using energy somehow like the guy in the GIF below), but the impact of these changes in the response to overfeeding remains to be determined. (5/n)

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pranea.bsky.social•44 days ago

What if part of the energy is being excreted during/after overfeeding? Well, fecal energy excretion actually decreased in response to overfeeding (at least during recovery)! Probably related to the fact that they are eating less (6/n)

pranea.bsky.social•44 days ago

Overall, this study provides new insights into the body's complex response to overfeeding. The ability to regulate food intake seems to be the primary defense against weight gain in mice. We are still very intrigued by the biological mechanisms behind this response! (7/n)

pranea.bsky.social•44 days ago

More research is needed to see if these findings apply to humans, but it offers relevant knowledge to better understand how our bodies respond to a calorie surplus. There is time-resolved energy expenditure data during and after overfeeding for the first time! (8/n)

pranea.bsky.social•44 days ago

This was a challenging study, where we had to fit our overfeeding setup into @sablesys.bsky.social metabolic cages, but eventually we made it work! :) Special mention to Charlotte Svendsen who mastered the overfeeding procedure quickly after I left the lab to start my PI adventure in Granada. (9/n)

pranea.bsky.social•44 days ago

The team was also composed of Camilla Lund (the master of mouse overfeeding), @lundjens.bsky.social, the amazing RMPP staff @metabolcenter.bsky.social (Christine, Morten, Thomas, Vibeke… and more!), @mkleinert.bsky.social with bomb calorimetry, and of course @clemmensenc.bsky.social (10/n)

pranea.bsky.social•44 days ago

Reviewers here were of great help! Thank you! The additional experiments they suggested definitely helped to create a more defined picture of the changes induced by overfeeding and allowed us to observe a subtle increase in energy expenditure with overfeeding. (11/n)

sablesys.bsky.social•44 days ago

Glad it worked for you & your colleagues! Sable's Promethion system is very friendly to custom setups (in part because it uses pull mode respirometry) & our support team will do everything they can to make your experiments successful. Even wildly successful, as in here!

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