3 reviews on planning, execution, & validation of transcriptomic experiments in neuroscience published in @natureportfolio.bsky.social #NatureNeuroscience.
A MUST READ if you're doing transcriptomics.
3 reviews, an amazing editorial, a beautiful cover – this is the issue of the year!
(mini thread)
A MUST READ if you're doing transcriptomics.
3 reviews, an amazing editorial, a beautiful cover – this is the issue of the year!
(mini thread)
Comments
Applying single-cell & single-nucleus genomics to studies of cellular heterogeneity & cell fate transitions in the nervous system
https://www.nature.com/articles/s41593-024-01827-9
Discover the power of sc/snRNAseq in unraveling cellular diversity/transitions. Highlighting creation of cell atlases for neuroscience.
https://www.nature.com/articles/s41593-024-01806-0
Review the strengths, limitations, & future opportunities of this tech. Essential reading for understanding cell interactions/gene regulation.
https://www.nature.com/articles/s41593-024-01814-0
Validation is key! This review dives into best practices for validating ssc/snRNAseq experiments. Learn about the challenges and solutions for ensuring robust and reliable findings.
What started as a discussion over lunch at @cshlaboratory.bsky.social #CSHLGlia2022, turned into 2.5 years of hard work & dedication from a massive team of seasoned & new experts
@adameykolab.bsky.social, Rajeshwar Awatramani, Trygve Bakken, Helen Bateup, Omer Ali Bayraktar, Aparna Bhaduri, @boyanbonev.bsky.social, Cathryn Cadwell, Emre Caglayan, @gocastelobranco.bsky.social, Fei Chen, Jerry Chen, Chintan Chhatbar, …
some things were missed out not due to a lack of importance or interest, but one paper turned into 3, and we couldnt have it expand to 5+!
importantly - we need to revisit these ideas regularly, & update with all the improvements that keep occuring
"While methods for the culture of CNS cells in the absence of serum have emerged, these are not always used"
Yup... this is going to be a gooooood (and honest) article!
I know its not the focus here, but I always think/talk about the wide use of serum in iPS cell protocols.