I am super excited to share our article addressing a longstanding question in neurobiology: how Ca²⁺ signaling cascades initiated at dendritic spines operate at a distance. Check it out here: authors.elsevier.com/sd/article/S... @cp-cell.bsky.social. - ThreadSky

lorenabenedetti.bsky.social • 68 days ago

I am super excited to share our article addressing a longstanding question in neurobiology: how Ca²⁺ signaling cascades initiated at dendritic spines operate at a distance. Check it out here: https://authors.elsevier.com/sd/article/S0092-8674(24)01345-X @cp-cell.bsky.social.

Comments

cohenlaboratory.bsky.social•68 days ago

Beautiful! Congratulations, Lorena and team!🎉

lorenabenedetti.bsky.social•68 days ago

Thank you so much, Sarah!

greenrat.bsky.social•68 days ago

📌 to read

lorenabenedetti.bsky.social•68 days ago

2/We reveal how a unique organization of ER-PM contact sites shapes the ER in dendrites into a ladder-like structure. These periodic ER-PM contacts act as local, spatial, and temporal amplifiers for Ca²⁺-dependent signaling.

lorenabenedetti.bsky.social•68 days ago

3/ Using live 3D lattice SIM imaging, we observed that along proximal and medial dendrites, longitudinal ER elements are connected by very regularly interspersed.
transverse ER elements.

lorenabenedetti.bsky.social•68 days ago

4/ These transverse ER elements protrude and form regular connections with the plasma membrane. Using volumetric EM reconstructions, we confirmed this in vivo in the mouse hippocampus.

lorenabenedetti.bsky.social•68 days ago

5/ and in neurons of the fly brain.

lorenabenedetti.bsky.social•68 days ago

6/ These junctions are populated by the ER-PM tethering protein JPH3.

lorenabenedetti.bsky.social•68 days ago

7/ We found that JPH3 plays a critical role in controlling the positioning and accumulation of voltage-gated ion channels at ER-PM junctions. These data reveal a new fundamental role for the ER in regulating the positioning of voltage-gated ion channels at the plasma membrane of dendrites.