WOW!!! Beautiful new Nature paper from Burkart Lab UCSD reveals how non-ribosomal peptide natural product synthetases work together.
They use tetrazine click chemistry to capture these huge protein complexes mid-reaction. 3 different snapshots show different states.
https://www.nature.com/articles/s41586-024-08306-y
They use tetrazine click chemistry to capture these huge protein complexes mid-reaction. 3 different snapshots show different states.
https://www.nature.com/articles/s41586-024-08306-y
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In 2006, as a grad student in Burkart lab we published in vivo carrier protein labeling using biorthogonal CoAs.
https://pubs.acs.org/doi/10.1021/ja063217n
That fall's ACS I met @jeremybaskin.bsky.social a Berkeley grad with an enthusiasm for cyclooctynes.
https://www.sciencedirect.com/science/article/pii/S1074552109000751?via%3Dihub
But the big dream - capturing massive NRPS complexes in action - remained stubbornly out of reach.
This sentence from the new paper 😬:
Scientific discovery requires both insight and persistence, and when they come together it is so lovely to see.
Congrats to the lab!! 🫡🫡