Amazing. It took Wiley, Bjorkmann, and colleagues hundreds of liters of cells to get enough MHC protein to crystalize. Now a small plate with one-step purification is sufficient.
I never went that far in literature for MHC, but I guess they were refolding from Ecoli inclusion bodies back then! Thank goodness never had to try that.
Even worse: 200 liters of non-recombinant human lymphoblastoid cells to get just 3 mg of protein. They probably needed like 50 preps to find crystallization conditions, and then metal derivatives. Inclusion bodies in E. coli are waaay high yield compared to that (50 mg per liter)!
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