A slight modification to the standard yeast lithium acetate transformation protocol increases transformation efficiency substantially.
Basically, 2 fold increase if you resuspend in 2M sorbitol after heat shock, and an additive increase with some sorbitol during heat shock.
Basically, 2 fold increase if you resuspend in 2M sorbitol after heat shock, and an additive increase with some sorbitol during heat shock.
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https://pmc.ncbi.nlm.nih.gov/articles/PMC11185651/
We skip the 30 min incubation at room temp and heat shock for 30 min.
Plates and our time are the priciest part of the transformation
We also sometimes have problems if the PEG or LiOAc are left ajar, so we do both in small bottles so we go through them fast.
Otherwise I'd be happy to share our protocols