🧵 Excited to share #Echidna, a Bayesian framework for quantifying the impact of gene dosage on phenotypic plasticity: tinyurl.com/296kf7hf! With @elhamazizi.bsky.social and @mingxz.bsky.social, we integrate scRNA-seq & WGS to uncover how CNAs drive tumor evolution and transcriptional variability. - ThreadSky

🧵 Excited to share #Echidna, a Bayesian framework for quantifying the impact of gene dosage on phenotypic plasticity: https://tinyurl.com/296kf7hf!
With @elhamazizi.bsky.social and @mingxz.bsky.social, we integrate scRNA-seq & WGS to uncover how CNAs drive tumor evolution and transcriptional variability.

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jlfan.bsky.social•72 days ago

📌 Why Echidna? Phenotypic plasticity (cell adaptability) is critical in cancer progression & resistance. But how much of this plasticity is driven by gene dosage vs external cues?
Echidna uncouples these effects, bridging genome & transcriptome across timepoints!

jlfan.bsky.social•72 days ago

How it works: #Echidna models single-cell RNA & bulk WGS data using a Bayesian hierarchical framework. Key features:
Deconvolves CNA profiles
Tracks clones & their phenotypic states over time
Introduces GDX: a metric quantifying gene dosage effects! 3/

jlfan.bsky.social•72 days ago

What did we find? Applied to tumor samples: 1️⃣ Clonal structure reconstructed with higher accuracy vs InferCNV/Numbat. 2️⃣ Temporal dynamics captured: tracks clonal evolution pre/post therapy. 3️⃣ Drivers of resistance identified using GDX in genomic hotspots. 4/

jlfan.bsky.social•72 days ago

A Case Study in Melanoma: 🧬 Clones resistant to anti-PD-1 therapy showed clusters of phenotype-defining genes in hotspots of amplification! Including:
S100 family and MHC-II genes. #Echidna also disentangles intrinsic (CNA-driven) vs extrinsic expression immune signaling!

jlfan.bsky.social•72 days ago

🌟 Why is this impactful? #Echidna doesn’t require joint scDNA/RNA sequencing (rare/expensive). Instead, it pairs scRNA-seq & WGS—readily obtainable from clinical samples. It’s scalable & can reveal mechanisms of cancer progression & treatment response! 🧬 6/

jlfan.bsky.social•72 days ago

📢 TL;DR: #Echidna 🦔integrates scRNA-seq & WGS to decouple gene dosage effects on phenotypic plasticity, tracks clonal evolution, and identifies resistance drivers in tumors.
Read more here: https://www.biorxiv.org/content/10.1101/2024.12.15.628568v1
Let’s unravel tumor evolution, one clone at a time! 7/7

aistockinsights.bsky.social•72 days ago

Wow, this sounds fascinating! Can't wait to learn more about your research findings. 🌟

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