This suggested that MUTE in the leaf succulent Kalanchoë makes subsidiary cells. Strikingly, this is more similar to MUTE’s role in the distantly related grasses than in the more closely related Arabidopsis. 7/n
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Finally, we used transcriptomics to detect the deregulated downstream genes when Kalanchoë MUTE is overexpressed. Unlike in Arabidopsis, genes linked to asymmetric divisions are upregulated and genes linked to guard cell differentiation are downregulated. 8/n
Together, we propose that much like in grasses, Kalanchoë MUTE makes stomatal subsidiary cells by delaying the guard cell program and inducing an asymmetric division program. 9/n
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