"Without the strawhat generational lice, life could not exist, and we would have no knowledge of Haki. They continually speak to us, telling us the will of D. When you learn to quiet your mind, you'll hear them speaking to you."
Impel Down sterilizes prisoners for diseases (by dipping them in boiling water), and Magellan wouldn’t take a risk with his prisoners catching lice unless he felt Luffy was clean. Meaning that he checked his hat for lice and found none.
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