#RNA enthusiasts, got a question for you..
How could one experimentally differentiate b/w a net increase in RNA synthesis vs. an issue with RNA export (like RNA getting stuck excessively in the nucleus)?
Are there tips/tricks in single nucleus seq or RNA-FISH etc to be able to delineate this?
How could one experimentally differentiate b/w a net increase in RNA synthesis vs. an issue with RNA export (like RNA getting stuck excessively in the nucleus)?
Are there tips/tricks in single nucleus seq or RNA-FISH etc to be able to delineate this?
Comments
- metabolic labeling with EU or 4SU
- measure the percent of intronic vs exonic reads similar to RNA velocity, more intronic reads implies more transcription while a decrease in intronic reads might imply nuclear accumulation of mature mRNA
- use another seq approach to look for chromatin / tx factor / etc changes and correlate with gene exp changes to test for txn changes