New paper on leaf heat tolerance in the next issue of Plant Biology. It challenges the assumed correlation between fluorescence-derived T50 measured soon after a temperature treatment, and leaf necrosis: onlinelibrary.wiley.com/doi/epdf/10.... The title basically is the abstract, but... (1/3) - ThreadSky

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martijnslot.bsky.social • 98 days ago

New paper on leaf heat tolerance in the next issue of Plant Biology. It challenges the assumed correlation between fluorescence-derived T50 measured soon after a temperature treatment, and leaf necrosis: https://onlinelibrary.wiley.com/doi/epdf/10.1111/plb.13732
The title basically is the abstract, but...
(1/3)

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martijnslot.bsky.social•98 days ago

This graph shows the decline in Fv/Fm measured 24 hours after leaf incubation (in green), yielding a T50 of ~49.5C. But the %necrosis 14 days after incubation (in red) did not increase until temperature exceeded 53C. Fv/Fm measured after 14 days does better (2/3)

martijnslot.bsky.social•98 days ago

Storing leaf disks for 2 weeks isn't practical for high-throughput heat tolerance. It defeats the purpose of measuring fluorescence as a proxy for necrosis.
It will be important to figure out what these parameters really tell us, and which parameters are most relevant for our questions (3/3)

loraxcate.bsky.social•98 days ago

Nice paper. Interesting findings. Do you think T50 can still be used to identify species that are more or less sensitive to heat stress? As a measure of relative sensitivity?

martijnslot.bsky.social•98 days ago

I think it absolutely can, but one would just have to be clear that it is the heat tolerance or heat sensitivity of PSII that is being evaluated (which is exactly what fluorescence based T50 gives you), without claiming that this T50 predicts the risk of leaf death.

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