(1st post @BlueSky) Preprint alert🚨a long thread. Cautions in the use of @nanopore sequencing to map DNA modifications: officially reported “accuracy” ≠reliable mapping in real applications. We performed a critical assessment of nanopore sequencing (across different versions of models) for the 1/n
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BREAKING news on my page-election was hacked
**Why**? In 2022 we reported in that 6mA is much less abundant in human cells than previously reported (mostly false positives). In 2023 we wrote a perspective in Nature Review Genetics to help navigate the common pitfalls for false positive in the mapping of DNA and RNA 3/n
@yimengkong.bsky.social (first author of the 2022 & 2023 papers, now a PI) and I 5/n
Why did we pick nanopore in this critical assessment? First of all, we have no bias among different sequencing platforms. In fact, we regularly use nanopore seq in our lab for 6/n