We teamed up with Alex Marson and Julia Carnevale’s Labs, who imaged TCR T cells co-cultured with RFP+ A375 tumor cells 3 ways: Safe harbor knockout (SH KO; control), RASA2 KO (Carnevale et al., 2022), CUL5 KO (Liao et al., 2024).
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Each well was imaged every 4 minutes at 10X magnification on Sartorius Incucyte for 72 hrs. Images include brightfield, RFP (cancer cell nuclei) channels.
With the masked, tracked cells, we went to work to develop Occident. We were curious how well the RFP markers captured cancer cell number; we found that RFP lags as a proxy for cancer cell numbers.
We found that the number of T cells attached to cancer cells reduces the likelihood that the cancer cell will proliferate, with the beneficial KO T cells having greater effects on proliferation reduction.
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