Cancer cell and T cell morphology changes dramatically depending on state. These changes are visible in the brightfield imaging – active interacting T cells are larger and change to less circular shapes. Cancer cell begin to aggregate together when interacting with T cells. - ThreadSky

thebeehive.bsky.social • 93 days ago

Cancer cell and T cell morphology changes dramatically depending on state. These changes are visible in the brightfield imaging – active interacting T cells are larger and change to less circular shapes. Cancer cell begin to aggregate together when interacting with T cells.

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thebeehive.bsky.social•93 days ago

While the # of T cell--cancer cell interactions increased similarly, these interactions & their effects were modulated by the CRISPR KOs. E.g., the time a T cell remained attached to a cancer cell (as estimated by a negative binomial and Markov model separately) was highest in RASA2 KO T cells.

thebeehive.bsky.social•93 days ago

Even more exciting, the speed of cancer cells decreased after interactions with T cells, as did their overall size (indicating stress).

thebeehive.bsky.social•93 days ago

Most thrilling is that we can identify active T cells based on relative cell size and morphology, and watch T cells activate (differentially based on condition) after interacting with cancer cells.

thebeehive.bsky.social•93 days ago

With a Markov model, we deconvolved when, in frame t-1, there is one cancer cell and one T cell in a window, and in frame t there is one cancer cell and two T cells. We were able to quantify how often this doubling of T cells attacking a cancer cell was due to proliferation or due to recruitment.

thebeehive.bsky.social•93 days ago

In summary, we found that, compared to the SH KO control condition, TCR T cells with the RASA2 KO have a longer dwell time and cripple cancer cells more effectively this way, whereas TCR T cells with the CUL5 KO proliferated more frequently upon activation, adding more T cells to the fight.

thebeehive.bsky.social•93 days ago

With five new collaborations in the works, and a paper characterizing the differences using explainable AI already accepted as an oral presentation at #PSB2025 (lead by high school senior Marcus Blennemann), look for future work in this space!
https://www.biorxiv.org/content/10.1101/2024.10.01.616134v1

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