Excited to dedicate our #FirstSkeet to our new #OpenAccess publication in Nature Communications revealing how #bristleworms make new #StemCells for #Regeneration of their body axis https://www.nature.com/articles/s41467-024-54041-3 ... (1/7)
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#Platynereis bristleworms grow by adding new segments at their posterior ends using a #StemCell zone. But what happens if the zone is cut off? First authors Alexander Stockinger and Leonie Adelmann @univie.ac.at used a time-resolved #SingleCell #scRNAseq approach to tackle this question... (2/7)
Almost 60 years ago, pioneering work by Dietrich Hofmann showed using histology that only hours after such amputations, cells adjacent to the wound undergo “re-embronisation”, exhibiting enlarged nuclei and nucleoli. This suggested a process modern stem cell biology calls “dedifferentiation”...(3/7)
...Indeed, our time-resolved molecular map revealed the emergence of #StemCell signatures from differentiated cells. When validating these signatures, we found that concomitantly, differentiation markers were down-regulated, whereas nuclei and nucleoli significantly increased... (4/7)
...Our #scRNAseq data revealed a rich set in cell-type markers, including novel markers for a mesodermal #StemCell population that appears to derive from a sub-epidermal layer upon amputation. It also led us to reveal a requirement for TOR signaling for regeneration.... (5/7)
...In complementary work, our collaborator Guillaume @balavoinelab.bsky.social and team pioneered a mosaic transgenesis method, demonstrating that mesoderm-derived and ectoderm-derived transgenic clones produce distinct offspring faithful to their original germ layer... (6/7)
Very grateful to all teams involved, to the Austrian Science Fund (FWF), the Agence Nationale de la Recherche (ANR), the Austrian Academy of Sciences (OeAW), the German National Scholarship Foundation and the University of Vienna @univie.ac.at for funding, and Paul Knabl for wonderful artwork. (7/7)
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