1) I don't know how you feel about antibodies, but here are a couple of (less-known) fascinating studies that might enhance your affinity towards these molecules One of these is Fab arm exchange This naturally occurs in vivo in IgG4 antibodies PMID: 17872445 bit.ly/Kolfschoten2... - ThreadSky

plasmacellguy.bsky.social • 97 days ago

1) I don't know how you feel about antibodies, but here are a couple of (less-known) fascinating studies that might enhance your affinity towards these molecules
One of these is Fab arm exchange
This naturally occurs in vivo in IgG4 antibodies
PMID: 17872445
https://bit.ly/Kolfschoten2007

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Comments

castltrastondrs.bsky.social•84 days ago

📌

immunerheum.bsky.social•71 days ago

So happy to connect on here! Love your paper summaries and history reviews!

plasmacellguy.bsky.social•71 days ago

Thank you :) for the very kind feedback.

I'm still enjoying it so will keep summarizing/reviewing

All the best with your work + life

plasmacellguy.bsky.social•97 days ago

2) Fab arm exchange is where antibodies swap half-molecules creating natural bispecifics
The third constant domain (CH3) (of the Fc) is essential for this
A later study showed aa positions 409 (in humans) as essential for his
R409 --> exchange
K409 --> stable
PMID: 21841137
https://bit.ly/Labrijn

plasmacellguy.bsky.social•97 days ago

3)This IgG4 property was later used in making bispecifics for other IgG isotypes for immunotherapeutic purposes
e.g.
PMID: 23479652
PMID: 25255089
PMID: 27216193
PMID: 34077951

plasmacellguy.bsky.social•97 days ago

4) Second IgG antibodies form hexamers on cell surface after binding antigen through noncovalent interaction via their Fc regions

This triggers the complement cascade

PMID: 24626930
https://bit.ly/Diebolder2014

Side-note: IgM is 100-1000 times better at activating complement than IgG

plasmacellguy.bsky.social•97 days ago

5) Transcytosis: of antibodies through cells (including blood-brain barrier and lung epithelium...etc) via polymeric Ig receptor (pIgR) on these cells
This explain early observations of lung epithelial cells shining with intracellular IgA
PMID: 781899
https://bit.ly/Soutar1976

plasmacellguy.bsky.social•97 days ago

6) The hinge region of antibodies determines its agonistic potential or lack thereof

e.g. human IgG2 (h2b isoform) enhances agonistic activities of Abs (through disulfide shuffling) independent of higher-order crosslinking via FcγRs.

PMID: 25500122
https://www.sciencedirect.com/science/article/pii/S1535610814004528