I recommend these optomechanics for 'O2-O3' in a single-objective light-sheet (SOLS) microscope: -> Mechanically and thermally stable. -> Relatively straightforward to align. -> 10 popular configurations. Make your own: github.com/amsikking/SO... Or buy it from @jsdaniel02.bsky.social at ASI! - ThreadSky

amsikking.bsky.social • 16 days ago

I recommend these optomechanics for 'O2-O3' in a single-objective light-sheet (SOLS) microscope:
-> Mechanically and thermally stable.
-> Relatively straightforward to align.
-> 10 popular configurations.

Make your own:

https://github.com/amsikking/SOLS_O2-O3_optomechanics

Or buy it from @jsdaniel02.bsky.social at ASI!

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amsikking.bsky.social•16 days ago

I'd like to thank @tanner-fadero.bsky.social, @jlazzaridean.bsky.social and @andrewgyork.bsky.social for their intellectual contributions. In particular I'd like to acknowledge @jlazzaridean.bsky.social for the significant effort she put into thermal testing and proof of concept.

amsikking.bsky.social•16 days ago

Thermal test data for objectives can be found in these repositories:

Nikon:
https://github.com/amsikking/Nikon_40x0.95air_focus_vs_temperture

AMS-AGY v1: https://github.com/amsikking/AMS_AGY_v1_focus_vs_temperature

AMS-AGY v2: https://github.com/amsikking/AMS_AGY_v2_focus_vs_temperature

amsikking.bsky.social•16 days ago

Okay so looks like .gif format isn't currently supported on @bsky.app! Let's try .mp4 then ;)

tanner-fadero.bsky.social•16 days ago

@amsikking.bsky.social I'm excited to try mine! Installation of the objectives snappy, and not having to worry about angle is a huge relief.

nano-juli.bsky.social•15 days ago

Cool! Thanks for developing and sharing.
How would you adjust the axial position of the objectives?

amsikking.bsky.social•15 days ago

As @tanner-fadero.bsky.social says the separation of O2-O3 can be adjusted with the mount holding O3. The position of the assembly (i.e. the position of O2-O3) can be fixed (1 way to build) or be moved as a whole (another way to build).

-> Either way it should be mechanical and thermally stable.

nano-juli.bsky.social•15 days ago

Thanks for the answers!
Since I have you here, is there any zenodo DOI or any way to cite the SnoutClub?
I would like to cite it in an article, as a proof of the supportive community of SOLS builders.

amsikking.bsky.social•15 days ago

At your service ;)

https://doi.org/10.5281/zenodo.14861825

https://github.com/amsikking/snoutclub

nano-juli.bsky.social•14 days ago

Thank you Alfred!! For the DOI and the support!

tanner-fadero.bsky.social•15 days ago

O3 is on a 3-axis polaris mount from @thorlabs.bsky.social: https://www.thorlabs.com/thorproduct.cfm?partnumber=POLARIS-K2T3

Which lets you translate O3 axially.

astormic.bsky.social•15 days ago

This is pretty cool!

How would you adjust O2 when checking its back-reflections?

amsikking.bsky.social•15 days ago

At least 2 approaches. 1) The O2-O3 assembly is fixed in place and you build the microscope around it. 2) You adjust the O2-O3 assembly to align with an existing optical axis.

-> In both cases the O2-O3 assembly as a whole should be mechanically and thermally stable.

tanner-fadero.bsky.social•15 days ago

O2 is your "diva." You define your optical axis as O2's optical axis. In practice, this means steering a pencil beam into the back of O2 with two mirrors until back-reflections look good.

dpshepherd.bsky.social•15 days ago

Super cool! Would be fun to play with at some point.

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