The hypothesis for this work was formed when we expectedly observed some Toxoplasma mutants with known fitness defects not showing defects in in vivo CRISPR screens. The results indicate that CRISPR screens can miss important virulence factors through paracine effects that function in the host.
Reposted from
eLife
In Toxoplasma gondii, a parasite infamous for manipulating the behaviour of its hosts that infects an estimated ⅓ of humans, genetic defects are rescued if co-infected with normal parasites, thanks to supportive secreted proteins.
https://buff.ly/3BtpD86
https://buff.ly/3BtpD86
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