Super excited to share that the final version of our study on the role of Adenylate Kinase 7 (AK7) on centriole maturation is finally out! @devbiol.bsky.social Read the full version here: www.sciencedirect.com/science/arti... - ThreadSky

alinegrata.bsky.social • 29 days ago

Super excited to share that the final version of our study on the role of Adenylate Kinase 7 (AK7) on centriole maturation is finally out!

@devbiol.bsky.social

Read the full version here:
https://www.sciencedirect.com/science/article/pii/S0012160625001344?via%3Dihub#fig1

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alinegrata.bsky.social•29 days ago

Primary ciliary dyskinesia (PCD) is a condition characterized by impaired mucociliary flow. Mutations in the AK7 gene have been found in patients, but AK7’s role in flow was unclear. We investigated this matter in flow-generating multiciliated cells (MCCs) in the skin epithelium of Xenopus embryos.

alinegrata.bsky.social•29 days ago

Loss of AK7 was related to PCD-like phenotypes, with decreased fluid flow and reduced beat frequency of MCCs. One of the challenges was to find a way to visualize live ciliary beating with a good enough spatiotemporal resolution (tipically cilia are around 15μm long with beating frequency of 15Hz).

alinegrata.bsky.social•29 days ago

We also performed SEM imaging and observed that ciliary structures are impaired when AK7 is lacking, with reductions in both cilia number and length.

alinegrata.bsky.social•29 days ago

The mechanism? Centrioles!
Cilia are anchored to the apical surface of MCCs via 150-200 centrioles, also known as basal bodies. These structures are generated close to the nucleus and then migrate apically, where they dock at the cell surface and subsequently give rise to cilia.

alinegrata.bsky.social•29 days ago

We observed that AK7 depletion leads to defects in centriole docking, with centrioles remaining sub-apical and therefore failing to nucleate motile cilia.

alinegrata.bsky.social•29 days ago

Our work suggests that although AK7 localizes at cilia, it has a non-ciliary function too, related to centriole maturation. We propose that the centriolar defects underlie much of the reduction in cilia number, contributing to the loss of mucociliary flow in AK7-depleted tissues, indicative of PCD.

venkyrao.bsky.social•27 days ago

That’s a cool video @alinegrata.bsky.social.

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