I wonder about the impacts beyond proliferation, such as in primary macrophage activation. Is this ITS supplement available Lucas? Very interesting work.
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Almost certainly many effects in many systems, which (to me) is exactly the point. Neither FBS nor defined media = physiological, but comparisons across them (+other environments) could be illuminating. Would macrophages proliferate or activate in defined media? We don't know...
but it would be interesting to find out! If they don't activate, then investigating the salient metabolic/signaling differences for why not could reveal new, manipulable biology. Or at least, that is the hope!
Re: ITS - the product number is in the methods. There are many "ITS" products available with different nutrient iterations, so check out our note about it (in the Methods) for more info. I would also be happy to discuss the details via DM/Zoom if you interested!
Comments
Almost certainly many effects in many systems, which (to me) is exactly the point. Neither FBS nor defined media = physiological, but comparisons across them (+other environments) could be illuminating. Would macrophages proliferate or activate in defined media? We don't know...