andrewleduc.bsky.social - Profile | ThreadSky | a Reddit-style client for Bluesky

Anyone out there working on single cell ribo seq, this is potentially an interesting alternative/complementary approach. There are some differences in the information they give and would be interesting to explore.

submitted 1 day ago • 0 comments

The aspiration to directly measure the 𝐫𝐚𝐭𝐞𝐬 of protein synthesis and degradation and control mechanisms of gene expression in the individual cells comprising mammalian tissues has always been a significant motivating factor for me to develop single-cell proteomic technologies. 1/n

submitted 2 days ago • 1 comment

How do different cell types regulate protein concentrations? Transcription is only part of the story! Our project focuses on better understanding the regulation of protein abundance by measuring transcription, translation, and protein clearance in single cells.

submitted 2 days ago • 0 comments

The talk by @andrewleduc.bsky.social at #SCP2025 is on YouTube: 𝐐𝐮𝐚𝐧𝐭𝐢𝐟𝐢𝐜𝐚𝐭𝐢𝐨𝐧 𝐨𝐟 𝐠𝐞𝐧𝐞 𝐞𝐱𝐩𝐫𝐞𝐬𝐬𝐢𝐨𝐧 𝐜𝐨𝐧𝐭𝐫𝐨𝐥 𝐢𝐧 𝐚 𝐦𝐚𝐦𝐦𝐚𝐥𝐢𝐚𝐧 𝐭𝐢𝐬𝐬𝐮𝐞 𝐚𝐭 𝐬𝐢𝐧𝐠𝐥𝐞 𝐜𝐞𝐥𝐥 𝐫𝐞𝐬𝐨𝐥𝐮𝐭𝐢𝐨𝐧 youtu.be/adkY6txDyqs?...

submitted 2 days ago • 0 comments

You ever just flip through genes for a while reading the functions on uniprot and just think, Wow cells do so much stuff

submitted 4 days ago • 0 comments

must be incredibly frustrating and disheartening to have federal funding that was promised to you for important work suddenly and arbitrarily ripped away

submitted 8 days ago • 644 comments

mitochondrial function, stress response, apoptosis, and autophagy experts, is this right

submitted 10 days ago • 0 comments

Congratulations Dr. Rahul Ragunathan on a successful defense @fanglab.org 🎉🎉

submitted 14 days ago • 0 comments

More evidence for the role of protein degradation in significantly shaping the proteome of brain tissue!

submitted 14 days ago • 0 comments

Julia presented remarkably simple and interpretable models from sophisticated measurements.

submitted 16 days ago • 0 comments

Multiplexed single cell proteomics to the moon 🚀 🚀

submitted 16 days ago • 0 comments

Amazing amount of progress made advancing high throughput proteomics in such a short time from the team at @parallelsq.bsky.social 🚀

submitted 16 days ago • 0 comments

We are excited to introduce ‘time’ as a new domain for proteomics multiplexing! It enables: -Label-free multiplexing -Combinatorial multiplexing with plexDIA Using combined 9-plexDIA and 3-timePlex we demonstrate 27-plex DIA 🚀

submitted 16 days ago • 1 comment

Read our new paper in Nature Comms: SPIED-DIA = spike-in enhanced DIA phosphoproteomics -> Boosts detection of key phosphosites -> Reveals JNK activation upon MEK inhibition in CRC cells -> Dual MEK/JNK targeting impairs growth 🔗 rdcu.be/enPF2 #proteomics #DIA #cancer

submitted 17 days ago • 0 comments

We have projects available on single-cell proteomics, including methods development, and applications to antiviral development and resistance. If you have a strong background in proteomics and are considering an MSCA, get in touch! marie-sklodowska-curie-actions.ec.europa.eu/news/msca-op...

submitted 19 days ago • 0 comments

So you’re telling us the radical transparency people are against… transparency? www.politico.com/news/2025/05...

submitted 22 days ago • 607 comments

The scientific literature is at risk of becoming flooded with papers that make misleading health claims. www.nature.com/articles/d41...

submitted 22 days ago • 2 comments

NEW: Under Trump, the National Science Foundation is awarding grants at the slowest pace in decades. We took a detailed look at every area of science affected by the funding lag. Here's a gift link: www.nytimes.com/interactive/...

submitted 22 days ago • 23 comments

When I was starting out modeling data, I always assumed that I needed to learn lots of fancy techniques and read different statistics text books to do useful analysis. However, intuition has gotten me much farther than time spent reading about complicated techniques.

submitted 22 days ago • 0 comments

Tune in to hear me give a talk on our work quantifying mechanisms protein abundance regulation in single cells from primary tissues!

submitted 24 days ago • 0 comments

This week, PTI will be sharing 4 preprints, including: ⬛️ New tools & mass tags for plexDIA ⬛️ 27-plex DIA enabling 500 samples / day ⬛️ Improved de novo sequencing of peptides ⬛️ Protein degradation dysregulation in Alzheimer's Check out @parallelsq.bsky.social for updates 🚀

submitted 25 days ago • 0 comments

Slow growth rates mean stable proteins have time to accumulate inside cells. The resulting consequence is that protein concentrations strongly depend on proteins degradation rates

submitted 26 days ago • 0 comments

𝐓𝐨 𝐮𝐧𝐝𝐞𝐫𝐬𝐭𝐚𝐧𝐝 𝐭𝐡𝐞 𝐛𝐫𝐚𝐢𝐧, we need to understand what shapes its molecular composition. What are the contributions of protein synthesis and degradation rates to determining protein abundance in the brain ? www.biorxiv.org/content/10.1...

submitted 26 days ago • 0 comments

@bsky.app Why are there no polls??

submitted 28 days ago • 0 comments

I just ran a sample that nearly out performed the state of the art in the beginning of my PhD 6 years ago, using 1/4000th the amount of sample in ~1/4th the time. That is pretty amazing.

submitted 35 days ago • 0 comments

We think this artifact may be more pronounced in single cell proteomics as compared to transcriptomics as proteins are a considerably smaller than the transcript that encodes them

submitted 39 days ago • 0 comments

This project started with an exciting discovery. As @andrewleduc.bsky.social and team interrogated deeper, we found artifactual contributions. So, we developed methods (scp.slavovlab.net/QuantQC) to reduce such artifacts. nature.com/articles/s41... rdcu.be/ekKTq 🧵

submitted 39 days ago • 1 comment

Our paper on the impact of protein leakage due to loss of membrane integrity for single cell proteomic sample prep is out in @natcomms.nature.com! We analyze thousands of primary single cells from murine tracheal tissue and record membrane permeability (sytox-green)🧵 www.nature.com/articles/s41...

submitted 39 days ago • 1 comment

The most profound insights are often also the most simple: growth-rate, by setting the dilution rate of intra-cellular molecules, controls the sensitivity of gene regulatory circuits. In retrospect it seems crazy that this effect seems to have been overlooked so far. www.science.org/doi/10.1126/...

submitted 45 days ago • 4 comments

Confounders are a major bottleneck to understanding biology. Interactions confounded by unobserved regulators (variables) cannot be causality inferred. Gazillion data points from the observed variables cannot compensate for unmeasured confounders. What can help ... 1/2

submitted 44 days ago • 1 comment