gavsturm.bsky.social
mitochondria, generative modeling, cellular aging, blender, procedural generation, complex systems theory. @WallaceMarshall @UCSF @mitopsychobio
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The transient nature of pearling makes it hard to detect - in normal cells you might need to sit and watch one spot for a long time and then, if you blink, you'll miss it
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absolutely! well ahead of its time and not well known enough: onlinelibrary.wiley.com/doi/10.1002/...
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yes but with suppressed curvature and reduced frequency.
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We hope you will enjoy these preprints. I will just close with this holiday wish to everyone who celebrates mitochondria: Happy Mito to all, and to all, a Good Mito!
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Thanks to my PhD advisor @wallaceucsf.bsky.social and all of our amazing collaborators, our biophysical model lays the groundwork for understanding pearling as a essential mitochondrial dynamic; integrating ionic flux, inter-organelle interactions, molecular motors, and bioenergetics. 10/10
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Studying this specific event goes all the way back to 1915, and has been given many names since. Take a close look at Margaret Lewis's hand-drawn mitochondria. However, a comprehensive model has been missing... 9/10
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The imaging data here includes over a 1000 videos of 4D light-sheet images. Crunching through this much image analysis was only possible thanks to @austin-lefebvre.bsky.social brand new automated organelle tracking platform, Nellie, 8/10
github.com/aelefebv/nel...
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How did we image this rapid event sometimes occurring within a second of time? thanks to @kayleyhake.bsky.social @andrewgyork.bsky.social & @amsikking.bsky.social @Calico we got to observe this biology with the best microscope in the word (imo), SNOUTY 7/10
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Other possible functions of pearling are emerging. We captured spontaneous pearling in diverse cell types including in important physiological events such neuronal action potentials, in T cell immune synapses, and in replicative senescence. There are so many exciting possibilities ahead! 6/10
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What is the functional utility of pearling? The brilliant investigation of @jclandoni.bsky.social .social and @sulianamanley.bsky.social establish mitochondrial pearling as the first and only known mechanism for mitochondrial DNA disaggregation and regular distribution. 5/10
bsky.app/profile/jcla...
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It's all physics. Using creative microscopic perturbations we establish that the 19th century Rayleigh-Plateau Pearling instability is the only model capable of explaining this event. This lead us to 3 physical forces; tension, pressure, and elasticity regulating pearling. Video by @Caleb_J_Rux 4/10
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You may be thinking of fission machinery. DRP1? a GTPase-like protein? but alas not quite! osmotic shock, ionophores, chemical fixation, membrane permeabilization, micro-needle force, laser-stimulation all induce mitochondrial pearling! we needed a more fundamental explanation…physics! 3/10
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Warning. Once you see it, you can’t unsee it. Fast spontaneous movements in which mitochondrial tubules transiently convert to thinly connected spheres (i.e. like a pearled necklace). So what is this strange event? 2/10
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5/ and in neurons of the fly brain.
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^simulated mitochondrial network made with blender geometry nodes
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count me in!
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would love to join as well