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jamesdmanton.bsky.social
Applied physicist & microscopist/microscopologist
335 posts 1,254 followers 523 following
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Posts 18 Comments 32

mScarlet3-M163H/M66Q #FluorescentProtein #GFP is reported as an exceptionally photostable red FP. I'm wondering if any of you have practical experience with it yet? @steveroyle.bsky.social @franbottanelli.bsky.social @joachimgoedhart.bsky.social @christlet.bsky.social @manorlaboratory.bsky.social

submitted 3 days ago • 4 comments

Multispectral imaging aficionados may be interested in the latest update to our multispectral experiment design tool, which includes some Cramér–Rao analyses (original derivation courtesy of Sjoerd Stallinga). Guide & more coming to a preprint near you soon. beryl.mrc-lmb.cam.ac.uk/calculators/...

submitted 10 days ago • 0 comments

For the past three years, I have been incredibly fortunate to collaborate with the outstanding scientific illustrator Alexey Chizhik (@alexeychizhik.bsky.social) on the cover designs for Biophysical Reports. His work is truly exceptional—be sure to check it out at alexeychizhik.com.

submitted 11 days ago • 1 comment

RMS Early Career Award: Applications deadline has been *EXTENDED* to 28 February! This is a great opportunity to receive recognition for your work - and a chance to win a cash prize 😀 Find out more and enter: rms.org.uk/resource/202... @rmsearlycareer.bsky.social @bioimaginguk.bsky.social

submitted 13 days ago • 0 comments

The self-driving multiscale microscope from @daetwylerstephan.bsky.social is out. It can image an entire zebrafish embryo longitudinally and follow a volume of interest over time with high resolution. We used it to study cancer-immune cell interactions. www.nature.com/articles/s41...

submitted 15 days ago • 5 comments

We present: Direct-view oblique plane microscopy www.biorxiv.org/content/10.1...

submitted 15 days ago • 4 comments

There have been some interesting recent preprints on photostable red FPs, so I thought I'd see how their photon budgets (in millions of emission photons) compare: EGFP: 1.0 StayRose: 3.8 mYongHong: 5.1 StayGold: 16.6 www.biorxiv.org/content/10.1... www.researchsquare.com/article/rs-4...

submitted 34 days ago • 2 comments

Our automatic parameter-free deconvolution code is now 2–3× faster while still producing exactly the same results as before. If you want to try it out, we have a Google Notebook implementation available at beryl.mrc-lmb.cam.ac.uk/rlgc_notebook/. Manuscript WIP.

submitted 36 days ago • 1 comment

Applications to our summer student training programme are now open. ☀️🔬 We’re looking for undergrad students to join us for nine weeks this summer. See the projects on offer and check the participation eligibility requirements on our website. ⬇️ www.crick.ac.uk/careers-and-...

submitted 43 days ago • 0 comments

Fellowships for international postdoctoral scientists to come and work in the UK.

submitted 43 days ago • 0 comments

Last few days to apply for this position. The LMB, is a wonderful place to grow a group with amazing, collaborative colleagues, core funding and a rich intellectual environment in Cambridge.

submitted 43 days ago • 1 comment

Reminder: Outstanding opportunity to open your group in the LMB’s Neurobiology Division as part of our Molecular Connectomics Initiative! You will be supported by core funding, excellent facilities and a lively international community of researchers. Apply by 19 Jan www.nature.com/naturecareer...

submitted 52 days ago • 0 comments

Many have asked how best to sample voxels in an OPM. Now Conor and Reto present a surprising answer...

submitted 62 days ago • 0 comments

At the risk of confirming that I'm a clueless physicist, how much of a transfection working without cell division is due to Pol II assembly in the cytoplasm versus dynein-mediated nuclear transport? Presumably CREB sites within the promoter are important for the latter mechanism?

submitted 71 days ago • 1 comment

How does nano-scale plasma membrane structure underpin tissue development? Let’s find out! First opportunity to do a PhD with me, co-supervised by Darius Koester, at Warwick. Fully funded studentship by #MIBTP and University of Warwick. 🔬✨🐟📸 More info here: www.findaphd.com/phds/project...

submitted 73 days ago • 0 comments

Exciting 3-year postdoc opportunity with @carter-lab.bsky.social within a programme aimed at identifying the links between microtubule motors and axonal cargos. Read more about the role here: www.nature.com/naturecareer... Apply by 30 DEC #ScienceJobs #CambridgeJobs #PostdocJobs #GenomeEngineering

submitted 77 days ago • 0 comments

Very happy that my work on developing multispectral live cell imaging technology has been recognised by the Cambridge Centre for Physical Biology! Thanks for the award!

submitted 78 days ago • 1 comment

Hello Bluesky! I am an engineer/scientist currently working at the MRC LMB in Cambridge UK. During my PhD I developed new imaging technologies to shed light on the dynamics of living cells e.g. here is a movie of 6 organelles in live human cancer cells. Follow for more updates! #science #biology

submitted 99 days ago • 1 comment