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jimhaseloff.bsky.social
Plant biologist at the University of Cambridge. Mashing up low-cost DIY tools for bioinstrumentation (biomaker.org) and engineering plant biology (openplant.org)
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3D print files for a collection of parts that allow micro servos to be plugged into Fischertechnik construction systems. Good for prototyping programmable mechanical devices... www.thingiverse.com/thing:2807112

The described procedure for making Lenticules is relatively simple. Cells are mixed with a solution containing a high concentration of trehalose, BSA and carboxymethyl cellulose as caking agents, saline (with optional food dye), and dried on parafilm before storage over desiccant.

However, details for preparation of Lenticules appears in a published patent: Storage of microorganisms, cells and tissue (WO 01/05941) See at: link.lens.org/aPFhU0Bjyli

Lenticules were described in doi: 10.1046/j.1365-2672.1998.00611.x. by Codd et al. - but composition of the critical component for storage, the "lenticulating fluid (Codds Medium)" seems missing in this and a subsequent paper (dot: 10.1046/j.1365-2672.2001.01432.x)...

Have always been interested in Lenticules and Vitroids (www.sigmaaldrich.com/deepweb/asse...) - as ways of storing/distributing microbial cells as stable, dry pellets, which could be widely useful for resource sharing. Lenticules originated at Public Health England, and a protocol is available...

Wild collection of heirloom and genetically variant seeds available from Tomato Revolution (www.tomatorevolution.co.uk) ready for next summer.

1/6 🧵 I'm building what might be the smallest #opensource #spectrometer with an easy to use UI! I call it a pocket spectrometer. Combining #M5StickC (~$20) with AS7341 sensor (~$16). My goal? A complete spectrometer that is barely larger than a sample cuvette itself.

Very cool use of optomechanical elements to make a "scanning" sepctrometer. blog.arduino.cc/2024/12/23/s...

Downloadable reference card for focus bracketing, on a site full of examples and advice about Olympus macro photography systems from Chris McGinniss www.chrismcginnis.com/gear

Here's an archived free copy of CL Strong's 1960 book with collected Amateur Scientist columns from Scientific American. Inspiration for citizen science. Forward by Vannevar Bush... archive.org/details/Proj...

Online portfolio of bryophyte plant images from across the globe - excellent macrophotography from Des Callaghan, captures some of the morphological diversity of this clade bryophytes.myportfolio.com/work

Fusion 360 files for 3D printing a good-looking Stevenson Screen (en.wikipedia.org/wiki/Stevens...) to house outdoor sensors and electronics, some OpenGreenEnergy. Nice design with other potential applications... hackaday.io/project/1650...

Successive updates/instructions for building solar-powered environmental sensing stations with custom electronics, wireless communications and 3D printed outdoor housing. hackaday.io/project/1650... and hackaday.io/project/1820...

Neatly designed $300 EDGE electrophoresis unit with integrated power supply and visualisation system from Edvotek - looks good for education and DIY lab - and design looks extendable www.edvotek.com/500

Collection of tips and techniques for handling delicate bryophyte plants for microscopy, including sectioning, KOH clearing, slide mounting, etc. from the Britsh Bryological Society www.britishbryologicalsociety.org.uk/learning/tip...

NIH 3D - large repository of 3D models for biomedical components (look under "Discover" and "Devices and Hardware" for lots of adapters, tube racks, etc for the lab) along with anatomical and molecular shapes... 3d.nih.gov

Tips for assembly of hybrid microscopy vessels - with embedded filters, fibre optics, coverslips, polystyrene windows, etc. - using colorFabb_XT co-polyester and Bio-PSA 7-4301 silicone adhesive for glue pubs.aip.org/aip/bmf/arti...

Useful set of guides for printing threads or embedding threaded inserts in 3D printed parts. formlabs.com/uk/blog/addi...

PlanktoScope: an open-source platform from studying plankton with high-throughput liquid sampling and quantitative imaging capabilities at a low cost (with clever reusable design for macro/micro imaging with HQ RasPi camera and twin M12 lenses) github.com/PlanktoScope... and www.fairscope.com

Build instructions for DIYNAFLUOR, a $40 fluorimeter for DNA quantification - uses 3D printed filter cube, LED source, TSL2591 sensor, cheap 10mm square bandpass filters - with full protocols for SYBR Safe dye measurements github.com/traulab/DIYN... and www.biorxiv.org/content/10.1...

Building an AI-powered artificial nose - could be useful for monitoring genetically regulated production of volatiles: makezine.com/projects/sec... and blog.benjamin-cabe.com/2021/08/03/h... and www.seeedstudio.com/Tiny-ML-powe...

CheesoSPIM. description of a $500 DIY mesoscopic light sheet microscope from Rusty Nicovich, with lots of interesting tips: cheap telecentric lens, hacked DSLR lens, cheap stage light filters for dichroics, optical clearing of wood... github.com/PRNicovich/c...

Original (?) report of spermine precipitation for highly selective precipitation of DNA, back in 1981 - based on polyamine collapse of DNA chains and great for excluding stubborn contaminants and obtaining clean DNA. Don't know why it is not more widely used... academic.oup.com/nar/article/...

Commercially available tiny microscope from the openUC2 team led by Benedict Diederich - a collaboration with Seeed studio. Open design, motorised focus stacking and extensible through the UC2 cage interface. www.seeedstudio.com/XIAO-Microsc...

See instructions for building variants of the tiny matchboxscope from Benedict Diederich and Vittorio Saggiomo, including the fluidiscope with built-in peristaltic pump: matchboxscope.github.io and matchboxscope.github.io/docs/Variant...

uConsole coming from Clockwork - including DIY assembled version. Interesting design for compact instrumentation, details at: www.clockworkpi.com/uconsole

Instructions for building a handheld computing device: Decktility by ByteWelder. Lots of neat details useful in other projects. bytewelder.com/posts/2023/0... and github.com/ByteWelder/D...

A online environment for playing interactively with reaction-diffusion systems from Karl Sims karlsims.com/rdtool.html

3D print files to build plant and animal cells for teaching and demonstration: www.printables.com/model/51034-...

Want to 3D print a pipette holder for Gilson and Eppendorf pipettes? New design by #IanKercher. www.printables.com/model/109822...

EEZ-Studio supports LVGL programming and interactive prototyping of touchscreen controllers. Should be useful for scientific instrumentation and research/educational work. www.envox.eu/wp-content/u...

EEZ Studio - a VERY interesting platform for programming touchscreens and microcontrollers. Cross-platform, open source, low-cost and no-code visual programming: www.envox.eu/studio/studi...

New device from Seeed Studio - SenseCAP Indicator with LoRa long range wireless support - along with 4" display, easy customisation with extra sensors and host of other features. Potentially useful for remote sensing: www.seeedstudio.com/SenseCAP-Ind...

Instructions for building a Raspberry Pi based mini data tablet. Uses 30% keyboard with some ideas for DIY data entry devices: hackaday.io/project/1952...

A range of interesting open hardware devices and parts from Jo Long and Will Dickson at IO Rodeo, including potentiostat and colorimeter and wide range of parts for customisation (iorodeo.com) - plus informative blog (blog.iorodeo.com)

Instructions for building a blue light transilluminator that can be used for real-time observation of DNA electrophoresis - full description, including custom circuit board from jorodeo on Instructables: www.instructables.com/Blue-LED-tra...

Clever use of off-the-shelf plastic drawers to build an agarose electrophoresis tank system - with some neat technical tricks - from Abizar on Instructables www.instructables.com/DIY-Simple-C...

Instructions for building a low-cost blue light DNA transilluminator by Gabriel St-Pierre. Simple 3D printed housing and LED strips. www.instructables.com/Blue-Light-D...

Statistics for Biologists: link back to a still highly relevant/useful collection of Nature Methods articles - a one-stop shop for stats tutorials: www.nature.com/collections/...

Large collection of extreme macro images from Achim Kluck, incl samples from (my favourite system) OM-1 with 90mm f3.5 lens 60mm, and more... www.flickr.com/photos/achim...

Frans (fotoopa), retired engineer and extreme macrophotographer - builds custom mechanics (Fischertechnik), electronics and optics for specialised high speed macro work. Collection of tools and images at: www.flickr.com/people/fotoo...

Another useful resource for optics - instructions for building your own LED illuminator. Might be used for custom microscopy or macrophotography journals.plos.org/plosone/arti...

Simple (and comprehensive) instructions for connecting plastic optical fibres to LEDs for cheap light guides. Handy for optical sensing... makezine.com/projects/how...

SacO2 Microbox containers for plant culture with hermetically closing covers and range of filters for best gas exchange, while minimising water loss. Definitely worth a try if you need alternative culture vessels. saco2.com/microbox-con...

News report of Des Callaghan's recent trip to document bryophytes in Tasmania. He shares his images freely - to inspire interest in these overlooked organisms. www.abc.net.au/news/2024-09...

Portfolio of lovely macro images of bryophyte plants across worldwide locations from Des Callaghan bryophytes.myportfolio.com

Collection of exquisite macro photographs from Linden Gledhill on Flickr - plants, animals, minerals. And some pics of the custom equipment used for capture. www.flickr.com/photos/13084...

Image collection of bryophyte-rich plant communities provided by Richard Droker - highly browsable library of micro-environments www.flickr.com/photos/wande...

The Kny posters are part of a huge collection of hi-res botanical wall charts offered by The Memory of Netherlands image library geheugen.delpher.nl/en/geheugen/...

LInk to a collection of extraordinary botanical posters produced by Leopold Kny at The Memory website in the Netherlands - includes iconic images of Marchantia geheugen.delpher.nl/en/geheugen/...