johannes-pilic.bsky.social - Profile | ThreadSky | a Reddit-style client for Bluesky

Join us at the ICSB in Dublin this October - I will chair the session on aging systems biology icsb2025.com

submitted 2 days ago • 0 comments

Interesting preprint about ATP gradients within cells: www.biorxiv.org/content/10.1...

submitted 5 days ago • 0 comments

Why do cortisol-lowering drugs work for Cushing’s but fail in mood disorders & chronic stress? We propose that the ability of the pituitary and adrenal glands to adjust their functional masses—normally beneficial—counteracts the drug’s effect. Using a math. model, we screened HPA interventions (1/2)

submitted 22 days ago • 1 comment

Great to see the mTurquoise2 plasmids that we've generated used as markers of subcellular structures rupress.org/jcb/article/...

submitted 27 days ago • 1 comment

🧵Prepint alert! Optimizing Multifunctional Fluorescent Ligands for Intracellular Labeling | tinyurl.com/3n55hvsc. With Jason Vevea, Ed Chapman, and @so-lets-kilab70.bsky.social, we combined dye chemistry, HaloTag, microscopy and cell biology to make protein purification and manipulation tools.

submitted 29 days ago • 2 comments

Mito-friends: What conferences are we attending this year? I ask because there are no mito/cyto symposia at my usual conferences, and it would be great to catch up! 🔬🧪

submitted 29 days ago • 0 comments

Our global study on the state of trust in scientists is now out in Nature Human Behaviour! 🥳 With a team of 241 researchers, we surveyed 71,922 people in 68 countries, providing the largest dataset on trust in scientists post-pandemic 👇🧵https://www.nature.com/articles/s41562-024-02090-5

submitted 37 days ago • 24 comments

Pericentromeric #heterochromatin clusters into chromocenters. @skrutlnize.bsky.social &co use quantitative mass spec to characterize the chromocenter proteome in multiple tissues, identifying a link between satellite DNA-binding proteins & #transposon repression🧪 @plosbiology.org plos.io/4akuNAi

submitted 41 days ago • 1 comment

FLIM imaging reveals IMM lipid heterogeneity in live cells. Find out more about this exciting tool: onlinelibrary.wiley.com/doi/10.1002/...

submitted 48 days ago • 0 comments

Stoked to share TWO preprints on mitochondrial pearling! 🎉 We uncover how spontaneous #mitochondria pearling drives #mtDNA nucleoid distribution (doi.org/10.1101/2024...), and @gavsturm.bsky.social et al. dissect the biophysics behind it (doi.org/10.1101/2024...). Details below! 🔬🧵🧪

submitted 65 days ago • 6 comments

2x Preprint drop!!! Mitochondrial pearling has arrived! “The Biophysical Mechanism of Mitochondrial Pearling”. “Pearling Drives Mitochondrial DNA Nucleoid Distribution” 1/10 www.biorxiv.org/content/10.1... www.biorxiv.org/content/10.1...

submitted 65 days ago • 3 comments

The dark side of fluorescent protein tagging: the impact of protein tags on biomolecular condensation www.biorxiv.org/content/10.1...

submitted 93 days ago • 10 comments

A highly photostable monomeric red fluorescent protein www.researchsquare.com/article/rs-5...

submitted 51 days ago • 2 comments

Interesting article: rupress.org/jcb/article/...

submitted 54 days ago • 0 comments

Vimentin-mSG FRAP

submitted 71 days ago • 2 comments

Struggling with proof-reading? A reminder that MS Word can read your document back to you via the 'read aloud' funciton found in the 'review' tab. An absolute game changer!

submitted 71 days ago • 1 comment

Oh, Switzerland, you pretty.

submitted 72 days ago • 0 comments

Looks interesting: www.sciencedirect.com/science/arti...

submitted 79 days ago • 0 comments

Supplementation of 1C precursors improve glucose clearance (self glucose tolerance test). Mechanism?

submitted 82 days ago • 0 comments

prelights.biologists.com/prelists/nov...

submitted 84 days ago • 0 comments

Tissue-specific adaptations to cytochrome c oxidase deficiency shape physiological outcomes 🧪 www.sciencedirect.com/science/arti... Congratulations to Milica Popovic and Lea Isermann from the Trifunovic lab for this nice work!

submitted 85 days ago • 1 comment

Very interesting approach to visualize proteins. www.nature.com/articles/s41...

submitted 86 days ago • 0 comments

Two cells photographed through a microscope. DNA in the nucleus and mitochondria in each cell are shown. #Science #Biology #CellBiology #microscopy

submitted 86 days ago • 0 comments

#Pearling in physiological neuronal axons!! 🤩📿

submitted 86 days ago • 0 comments

My turn to create a starter pack for scientists using live imaging in their research! Of course, I’ve likely missed many—please let me know who else I should include. Thanks! go.bsky.app/KYYr7xc #liveimaging #livevellimaging #microscopy

submitted 98 days ago • 113 comments

Hey blue skies- We have updated our latest #BioRxiv paper- We found that #mitochondria in #plants without mtDNA move and connect differently to those with mtDNA! Now featuring two different methods of measuring mtDNA and mitochondrial motility. Check it out: www.biorxiv.org/content/10.1...

submitted 103 days ago • 0 comments

Cool new work from the lab by @chris-morrow.bsky.social: Often we think of auto-fluorescence as a hindrance to experiments - but endogenous metabolites have specific fluorescence we can use to determine the metabolic state of the cell - and predict lifespan.. www.nature.com/articles/s42...

submitted 96 days ago • 3 comments

posting here since bsky is taking off! nature.com/articles/s41586-024-08146-w Congratulations to my good friend @keunwooryu.bsky.social and the Craig Thompson lab on uncovering metabolically distinct mitochondrial subpopulations. Really cool cell biology Keun!!

submitted 96 days ago • 1 comment

Finally moved to #Bluesky and happy to share our recent work! We found that hexokinase 1 rapidly forms rings around #mitochondria during energy stress to regulate mitochondrial fission: www.cell.com/molecular-ce... For a quick summary of our findings, check out the comment section.

submitted 95 days ago • 2 comments

Hey people! @florentwaltz.bsky.social was kind enough to make a pack for mito enthusiasts. Check it out and join/follow for updates on the most interesting organelle!

submitted 96 days ago • 0 comments

Visualizing mitochondrial membrane proteins in live cells can be challenging due to the size of fluorescent protein tags (~240 residues). In our recent paper, we show how a small tetracysteine tag (6 residues) enables accurate visualization of VDAC1 in live cells. journals.plos.org/plosone/arti...

submitted 95 days ago • 0 comments

How do #mitochondria decide when to divide or fuse? I’ve always thought mtDNA played a key role in this process, so I was very surprised by this finding: The loss of mtDNA does not affect mitochondrial fission and fusion rates. www.molbiolcell.org/doi/10.1091/...

submitted 95 days ago • 0 comments

Textbook oxidative phosphorylation needs to be rewritten with Fernando Abdulkader www.sciencedirect.com/science/arti... Free for 50 days here: www.sciencedirect.com/science/arti...

submitted 97 days ago • 1 comment

Trying to gather all the mitochondriacs that landed here Help me fill up this starter pack! Just ask and i'll add you 🤓 #Mitochondria #Mitochondriacs #Respiration #Organelle go.bsky.app/G1DPArc

submitted 100 days ago • 18 comments

🔬 Curious about #mitochondria #imaging but unsure where to begin? Our review might help! 🚀📜🧪 www.annualreviews.org/content/jour... #Quantitative #Microscopy #SuperResolution #AcademicSky #ScienceSky #CellBiology #mtDNA

submitted 101 days ago • 7 comments