juliecocquet.bsky.social
PI @Inserm @InstitutCochin, Paris. Gene regulation, chromatin remodeling, male germ cells, paternal epigenome, sex chromosomes & genomic conflicts.
21 posts
568 followers
304 following
Prolific Poster
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She will present her work at our next symposium in May 26-28th. So don't forget to register! 👇 gdr-repro.cnrs.fr/les-evenemen...
Early birds until February 14th
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Thanks Sandra!
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Thanks!
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Thanks Peter !
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5/5 Work from our former PhD student in bioinformatics Manon Coulée cosupervised by L El Khattabi and I. The model was validated by elegant spike-in cut&tag by @albiglero.bsky.social
Thank you to our ⭐️ collab. Pierre Fouchet, Lucia Alvarez-Gonzalez and @aruizherrera.bsky.social
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4/5 This relates with the fact that, in male germ cells, the chromatin of the X differs from that of autosomal chromatin as a consequence of Meotic Sex Chromatin Inactivation (MSCI).
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3/5 We show that the genes repressed and activated by DOT1L are in distinct chromatin environments: repressed genes are in H3k27me3+ regions while activated genes are in H3k27ac+/H3k79me2+ regions. Interestingly many DOT1L-activated genes in spermatids are on the X chromosome...
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2/5 By integrating RNAseq spike-in and ChIPseq data from 7 marks (including H3k79me2) we investigated the chromatin environment of deregulated genes in spermatocytes I and round spermatids.
#chromHMM
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🧵
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In brief we show that despite the fact that H3k79 methylation is associated with gene expression, the H3k79 methyl transferase DOT1L represses more genes than it activates in male germ cells.