madic.bsky.social
Shooting electrons, ions and photons (mainly) at plants to study cell-cell communication @mpibiochem.bsky.social & @hhu.de
159 posts
2,205 followers
1,389 following
Regular Contributor
Active Commenter
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She qualifies for US Secretary of Health 👍
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Soon!:)
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I keep coming back to these. Still relevant and unmatched.
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Thank you Pascal! Plant samples do make the efforts worthwhile 😜
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Thanks a lot Ben, also for all the support along the way!!
Yeah, Physco is indeed deserted (no tumbleweed emoji?!). The Arabidopsis embryo gets quite crowded though.
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Hey Florent,
thanks a lot! This definetly took the combined Martinsried effort; including your expat feedback. Curious to see what you are cooking up with your LO system, expanding the range of green samples 🌾💚
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Thanks Yasin! Maybe it's time to catch those cell-to-cell traveling NBR1s in action 😏
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Thanks a lot, Michael!
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A step-by-step protocol, as well as the biological stories (including on #Plasmodesmata) will follow soon. Happy lifting #teamtomo🏋️
🧵5/5
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Workflows are also applicable to thicker tissues. Examples of #Arabidopsis embryo and epidermal salt glands of the halophyte Limonium bicolor are included. 🧵4/5
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Subtomogram averaging and spatial analysis of #Rubisco in #Physcomitrium as proof of principle. 🧵3/5
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We use autofluorescence for label-free targeting within high-pressure frozen samples, followed by serial LO. 🧵2/5
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*record ;)
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Amazing album!
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Do you have a planty hand sign to go with it?
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How well does it purify? X-ray needs mg. If you don't have much, better to put it on a grid.
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"Catching on fire" means full of bots or people being excited?
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Yes, I hope long-term there will be a better implementation into bsky app, not relying on third party software of require you to set up a server yourself. IMO its the biggest strength of bluesky and should be more userfriendly. There is the generic "Popular with Friends" feed everyone has access to.
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With feeds, you can build your own algo timeline. Filtered by keywords or accounts. Sorting by engagement metrics possible too.
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go.bsky.app/BXnLeZ2
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I tried GPT for sequence analysis. It was doing reasonably well on predicting topology, presence of signal peptides and finding unusual patterns. However, when asked something like "output the sequence of the transmembrane region" it would hallucinate/mix up residues.
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Will be my first!🕺
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Thanks for the link. Their lumenal reporter protein (LRP) is 2-4nm with pI=4.3.
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Dang. I was hoping cryoET was going to be something else.
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Thanks! I do like his laser analogy. Though, the pop-out effect of capitalized forms in a wall of text may help readability.
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We dont know yet. Difficult to resolve. Since curvature is believed to be maintained by reticulon/reticulon-like proteins, reducing the protein concentration should decrease curvature. Or maybe that is to simplistic?
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True! Wouldn't know how to adress flow through DT experimentally though. Assuming its bilayers, the lumen diameter is <5nm (more like 3nm in our data) with anionic walls. Cannot imagine free diffusion through that. Maybe thats why it goes apoplasmic rout with local repeaters?
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Thanks a lot!
So, although tubulating proteins are similar, ER diameter varies. The 25-90nm in axons still allow calcium flow, while 10-15nm in plasmodesmata do not (www.science.org/doi/10.1126/...).
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Great question. How does it work in axons; can you recommend a read?
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Agreed. Though, I see little to no advantage in MDPI & Co over pre-prints. The Rxivs are free and "If you trust the science and scientists" still applies.
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Not necessarily a bug. Feed creators can set how to order posts. Try making your own feeds:) probably one of the best features on here
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It's gorgeous! Perfect tomo slice in the figure too.
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but..
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Same for most stipends
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If you want to make your own without setting up a server:
blueskyfeedcreator.com is easy to use and running very smooth recently