nano-juli.bsky.social
Physicist and dad. Developing photonics tools for plasmonic chemistry and nanothermometry at UNSAM.
Drosophila brain imaging by #SnoutClub.
Photons are my own.
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194 following
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It's been a long journey, only possible thanks to my lovely co-authors who are not (yet) in Bluesky.
Big thanks also to @amsikking.bsky.social , @andrewgyork.bsky.social and
@lumasullo.bsky.social, they have always been there to help.
Stay tuned for new imaging on living Drosophila!
6/6
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In addition, we can do 2 colours imaging...
Here the membrane is labelled in green (GFP), and dense core vesicles are labeled in magenta (mCherry).
We also show mitochondria imaging in the manuscript.
5/6
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And what about the photobleaching?
We can image the fly for 30 minutes without bleaching it, and can get 10 times more images than with confocal microscopy!
Also, we don't rely on phototoxic pulsed infrared lasers like in 2-photon microscopy.
4/6
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We imaged the axonal projections of small lateral ventral neurons (known as s-LNvs) in living adult flies.
The NA=1.1 allowed high spatial resolution.
Here, a line intensity profile showing features as small as 360 nm.
3/6
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We used a Single Objective Light-Sheet (#Snouty, for the friends).
It is less invasive than a lattice light sheet because it requires a smaller dissection area on the head´s cuticula, as it is only necessary to accommodate the light cone of a single objective. And it is simpler. 2/6
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Oh no! Keep us posted on your new adventures.
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For 2-3 color videos doesn't work that well. Any solutions are appreciated
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Beautiful!
How do you go from .tiff to bluesky video? Is it a .gif?
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Thank you Alfred!! For the DOI and the support!
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Thanks for the answers!
Since I have you here, is there any zenodo DOI or any way to cite the SnoutClub?
I would like to cite it in an article, as a proof of the supportive community of SOLS builders.
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Cool! Thanks for developing and sharing.
How would you adjust the axial position of the objectives?
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Hi Falko, how can I join that group?
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El grupo de Nanofotónica está liderado por Ianina Violi y Julián Gargiulo, quienes dirigen a la tesista doctoral María Cristina Mina y supervisan el trabajo de tres estudiantes de la licenciatura en Física de la UBA: Abril Pereyra, Valentín Salari e Iñaki González Vandam.
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El grupo de Nanofotónica trabaja en el laboratorio de láseres del INS. Fabrica dispositivos que permiten manipular la luz en la nanoescala, y tienen muchísimas aplicaciones como biosensores ultrasensibles, en celdas solares para la producción de combustibles o microfluídica.
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🧐Poder hacer este tipo de arreglos es muy útil para el estudio de nanomateriales, ya que una vez que están ordenados de a uno se les pueden medir propiedades a nivel de partícula única, mucho más ricos en información que cuando se estudian todas las partículas juntas en solución
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🔬Usamos una impresora óptica de nanopartículas, que permite usar la técnica de pinzas ópticas en la que un láser toma las nanopartículas una por una y las posiciona sobre un vidrio hasta crear un arreglo, en este caso el famoso librito de nuestra universidad.
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Thanks for the support!
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Welcome!
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Me cambio la calidad de vida. Un día sin ella y la abstinencia es muy fuerte.
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Beautiful!, what's the LUT?
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My #BioimageTooloftheDay is the NeuroCyto Luts Fiji plugin by @christlet.bsky.social
A must have collection of very useful LUT to make beautiful merge images
forum.image.sc/t/neurocyto-...