ulrichschwarz.bsky.social
Theoretical biophysicist who loves to talk about science.
Homepage https://www.thphys.uni-heidelberg.de/~biophys/
34 posts
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Great work, congrats!
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Congrats Melina, Petr and team!!!
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Kirsty, please let me join, too🙏🏻
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(5/5) Coat stiffening could result e.g. from incorporation of new molecules or from rearrangements inside the clathrin coat. Our analysis agrees with the emerging notion that clathrin coats are rather plastic. For more details, please read our paper
doi.org/10.1103/Phys...
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(4/5) We therefore suggest that the coat stiffens dynamically as invagination proceeds. This results in a downhill energy landscape.
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(3/5) We next combined the consensus pathway with the standard continuum model for the energetics and found that the bending stiffness of the clathrin coat introduces a very high initial energy barrier.
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(2/5) We first analyzed data from several high-resolution imaging techniques and found that they are all fitted pretty well by the cooperative curvature model (introduced earlier in Mund et al., Journal of Cell Biology 2023). We call the average scenario the "consensus pathway".
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Please add me to your list. Thank you!
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Please add me, too. Thank you
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Please add me to your list. Thank you!
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Please add me, too. Thanks!
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(9/9) Many thanks to Lukas Riedel, Valentin Wössner and Dominic Kempf for making this challenging project work, and to our cluster of excellence STRUCTURES for support. Read the full paper here: doi.org/10.1016/j.jm...
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(8/n) It took some while to figure this out, but in the end it worked very well. Given the high-level approach taken here, the results are surprisingly good, showing close resemblance with experimentally observed stress fibers configurations:
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(7/n) To simulate the statistics of many stress fibers and to combine them with genetic algorithms as optimization scheme, we implemented them in the #FEM software environment DUNE www.dune-project.org
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(6/n) Discussing this question in our cluster of excellence STRUCTURES with Peter Bastian from applied mathematics, we found an answer: contractile beams embedded in a bulk elasticity can be efficiently simulated with the cut finite element method doi.org/10.1007/978-...
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(5/n) It is hard to model this, so we were thinking about a functional approach: what if we only asked how to position stress fibers in order to avoid mechanical stress in the cell? Stress can be calculated in continuum mechanics, but what is a good model for a stress fiber in continuum mechanics?
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(4/n) The mechanisms underlying stress fiber formation are very complicated and involve many molecular players, including actin, non-muscle myosin II, tropomyosin, a-actinin and zyxin, as reviewed e.g. here doi.org/10.1242/jcs....
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(3/n) For example, in this cool experiment using 3D-printed scaffolds it has been shown that stress fibers form quickly when cells are repeatedly stretched at one adhesion point doi.org/10.1016/j.bi...
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(2/n) Stress fibers have many functions in adherent cells, but one main purpose seems to be to protect them from mechanical damage and to ensure structural integrity, because they often form in stressful situations, as reviewed e.g. here doi.org/10.1242/jcs....
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Thanks for the comment, Ben. Indeed this account was automatically suggested by Sky Follower Bridge. Now I follow your account on mechanobiology.