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zenlabpasteur.bsky.social
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New call for research Group Leaders at Institut Pasteur Paris !

submitted 81 days ago • 0 comments

Just published in Development (@biologists.bsky.social): doi.org/10.1242/dev....

submitted 95 days ago • 2 comments

On Mon 18/11, we held a mini symposium on the novel perspectives in human embryo research @institutpasteur. Lively discussions thanks to our prominent speakers/chair Thanks to Nicolas Rivron; Alain Chédotal; Cécile Martinat; Ken McElreavey; Bernadette De Bakker and the chair Alfonso Martinez Arias

submitted 99 days ago • 0 comments

This also draws yet another parallel between physiological regulation stem cell pools and oncogenesis, which has been referred to as a process through which cells become “asocial” and cease to coordinate with their neighbors (18/18)

submitted 102 days ago • 0 comments

Although we were not able to identify what advantage this confers for NSC homeostasis, this non-canonical maintenance of Notch effectors illustrates different scales of quiescence regulation with some cells seemingly being able to listen less to their neighbors (17/18)

submitted 102 days ago • 0 comments

In vivo experiments confirmed that expression of nr2f1b in zebrafish NSCs is associated with a weaker response to Notch inhibition and functional experiments with morpholinos or electroporations of nr2f1b confirmed its ability to decrease sensitivity to Notch inhibition. (16/18)

submitted 102 days ago • 0 comments

Interestingly NR2F1 had previously been identified as a regulator of Notch signaling in the mouse inner ear (journals.biologists.com/dev/article/...), with downregulation of Notch effectors in NR2F1 knock-out mutants (15/18)

submitted 102 days ago • 0 comments

Using a gradient boosting method derived from SCENIC applied to both control and treated cells, we identified NR2F1/Coup-TF1 in sillico as a putative regulator enabling this resistance to Notch inhibition. (14/18)

submitted 102 days ago • 0 comments

Finally, we also identified that one cluster of cells barely responded to Notch inhibition, maintained expression of Notch effectors and did not upregulate genes associated with NSC activation. (13/18)

submitted 102 days ago • 0 comments

Although there are obvious differences between parenchymal astrocytes in mouse and astrocyte-like RGs in zebrafish, this suggests that comparative studies focusing on these cells might help better understand how to promote brain regeneration. (12/18)

submitted 102 days ago • 0 comments

The reported properties of the induced activation of these cells indeed shared similarities with that of astrocyte-like NSCs in the zebrafish pallium, such as participation in regeneration or reliance on Notch signaling (11/18)

submitted 102 days ago • 0 comments

Harmonizing the different reports of cell classification led us to consider that the cells that had been described as latent NSCs under different names in different publications were in fact likely the same population of parenchymal astrocytes close to the lateral ventricles. 10/18

submitted 102 days ago • 0 comments

Using the set of genes which we previously identified as allowing for distinction between murine RGL and astrocytes, we reclassified qNSC1 cells as astrocytes, in accordance with scRNAseq and in situ data from A Cebrian-Silla and methylome data from LPM Kremer. (9/18)

submitted 102 days ago • 0 comments

We found that the distinction between qNSC1 and qNSC2 had not been made in most cases even when the cells belonging to each group were present in the dataset. (8/18)

submitted 102 days ago • 0 comments

To get a clearer picture we re-analyzed scRNA-seq datasets encompassing the SVZ, generated by the labs of F Doetsch (@doetschlab.bsky.social), A Martin Villalba, J Frisen, S Linnarsson (@slinnarsson.bsky.social), J Biernaskie, N Rajewsky, F Miller, A Alvarez Buylla and O Raineteau. (7/18)

submitted 102 days ago • 0 comments

A similar phenomenon had been described in mouse astrocytes and putative subventricular zone (SVZ) radial glia-like (RGL) (called qNSC1) which respond to injury. (6/18)

submitted 102 days ago • 0 comments

In particular we found that one group of NSCs, recently described as being very similar to mouse parenchymal astrocytes, is efficiently recruited by Notch inhibition leading to the appearance of a cell state defined by co-expression of astrocytic and activating NSCs markers. (5/18)

submitted 102 days ago • 0 comments

With scRNAseq after 24hours of Notch inhibition we found that most cells had responded, but not in the same way. (4/18)

submitted 102 days ago • 0 comments

We attempted to push NSCs out of quiescence using Notch inhibition to see if previously identified clusters reacted differently to a pro-activation stimulus. (3/18)

submitted 102 days ago • 0 comments

Drawing from previous characterization of the heterogeneity of adult Neural Stem Cells (qNSCs) in the zebrafish pallium and the prediction that some of that heterogeneity comes from differences in quiescence depth (2/18)

submitted 102 days ago • 0 comments

New pre-print from the lab out on @biorxivpreprint (www.biorxiv.org/content/10.1...). 1/18

submitted 102 days ago • 0 comments