Our latest work: how can compartmentalization emerge in a eukaryotic genome lacking canonical heterochromatin? By investigating bacterial genomes put in yeast, we show that the presence or absence of transcription is sufficient! #chromatin #3Dgenome #generegulation www.science.org/doi/10.1126/... 👇 - ThreadSky

rkoszul.bsky.social • 117 days ago

Our latest work: how can compartmentalization emerge in a eukaryotic genome lacking canonical heterochromatin?
By investigating bacterial genomes put in yeast, we show that the presence or absence of transcription is sufficient!
#chromatin #3Dgenome #generegulation
https://www.science.org/doi/10.1126/science.adm9466
👇

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oespeli.bsky.social•117 days ago

Great work! Congrats Léa, Christophe, Jacques and Co.

rkoszul.bsky.social•117 days ago

Using MNAse, ChIP, ATAC, CRAC and RNA-seq, Hi-C and imaging, we characterized the composition, activity and folding of bacterial genomes of varying sequence composition introduced as supernumerary chromosomes into budding yeast. Depending on their GC%, their fate was surprisingly different.

rkoszul.bsky.social•117 days ago

Sequences close to the yeast GC are highly transcribed, somehow following the orientation of prokaryotic genes, and mix with yeast chromosomes.
In contrast, a rich AT bacterial genome is not transcribed and forms a globule at the nuclear periphery, independently of SIR heterochromatin formation...

rkoszul.bsky.social•117 days ago

Dispersed over several chromosomes, this inactive compartment is maintained until mitosis when cohesins compact all chromosomes similarly.
Reducing transcription eliminates compartmentalization, with all yeast chromosomes now resembling the bacterial chromosome...

rkoszul.bsky.social•117 days ago

Deep learning models (@chromozz.bsky.social) trained only on yeast chromosomes predict nucleosome positioning, RNA Poll II and cohesin tracks along foreign DNA, based on the sequence alone. This implies that the behavior of any DNA in a host cell follows deterministic sequence-based rules.

rkoszul.bsky.social•117 days ago

This result suggests that simple sequence-dependent features could have contributed to the emergence of bipartite compartmentalization of genomes during evolution, either by sequestering inactive genetic material or by facilitating the functional integration of active sequences.

rkoszul.bsky.social•117 days ago

All these results and much more can be found in our paper, a massive work led by @leameneu.bsky.social, @christophechapard.bsky.social, and Jacques Serizay who generated all the beautiful analyses. Thanks to all the contributors, esp. @chromozz.bsky.social and Alex Westbrook for the deep-learning!

mathienz.bsky.social•117 days ago

Great work! 👍🏼👏🏼

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