How do you study gene-environment (GxE) interactions in humans? Well, there's probably no way to avoid studying each exposure individually, but what about the cellular context? If you choose directed differentiation, you have to study GxE one cell type at a time. If you choose an organoid system - - ThreadSky

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ygilad.bsky.social • 83 days ago

How do you study gene-environment (GxE) interactions in humans? Well, there's probably no way to avoid studying each exposure individually, but what about the cellular context? If you choose directed differentiation, you have to study GxE one cell type at a time. If you choose an organoid system -

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ygilad.bsky.social•83 days ago

following by single cell sequencing, you can characterize GxE in many cell types, but organoids take a long time to establish, are expensive, and are difficult to dissociate. The GxE framework requires collecting data from a population sample, which makes it quite challenging to -

ygilad.bsky.social•83 days ago

work with organoids. We propose a new model - heterogeneous differentiating cultures (HDCs). These are guided (rather than directed) cultures that establish a complex and heterogeneous collections of cell types. We recently reported the properties of a cardiac HDC version:

ygilad.bsky.social•83 days ago

cell culture that can be established in less than 2 weeks, and includes practically all the cardiac cell types that can be found in the heart, or in a 3 months' old cardiac organoid. The cardiac HDCs are not a good model for studies of heart physiology and function, but may be a great mode for -

ygilad.bsky.social•83 days ago

studies of regulatory variation and GxE interactions in the heart. here's the preprint: https://www.biorxiv.org/content/10.1101/2023.07.21.550072v4

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