adamkglaser.bsky.social
Developing hardware + software tools for large-scale imaging at the Allen Institute for Neural Dynamics
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None of the above captures the long WD (35 mm). Perhaps we should introduce a volumetric SBP... that divides the usable WD by either the Rayleigh axial resolution, or an isotropic resolution assumption that divides by the lateral resolution -> would be very high for this lens.
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Thanks for resolving the numbers.
Agreed, this was the primary motivation for ExA-SPIM in the first place. More pixel parallelization with a larger SBP, either in 2D (global shutter) or even along 1D for rolling shutter (i.e. for ASLM). Also removes some scanning bottlenecks with a low FPS sensor.
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That is right, I used the equation from doi: 10.1117/1.ap.3.4.044001. The required number of samples to fully resolve the signal - also their equation uses 0.5 instead of 0.61. Lens has more information than any current standard camera sensor but potentially could be fully used in the near future.
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Totally agree with you 😆 space-bandwidth product > etendue (!)
For 420 - 850 nm light the SBP is 175 - 825 megapixels. Around 500 megapixels at 500 nm.
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It’s across the board. All grants from all agencies, future ones on pause and current ones also on pause :/
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Can maybe estimate based on the 35 mm full frame sensor size (36x24 mm). So probably around 1.46 micron pixels? The max rate is 3280 megapixels/sec 🤯