axonsalex.bsky.social
Developmental Neurobiologist at UZH | Microscopy Enthusiast. Using the chicken embryo to decipher the molecular mechanisms of neural circuit formation. Fascinated by axonal growth cones, primary cilia, and extracellular vesicles.
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cool!
here are chick commissural axons crossing the neural tube floor plate :): bsky.app/profile/axon...
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Hi Erica,
thanks!
for 2D cultures: journals.biologists.com/jcs/article/...
for 3D cultures: www.frontiersin.org/journals/mol...
for live imaging I used 8-well IBIDI μ-slides with same PLL/Laminin coating and the same medium as in the JCS paper.
Cheers
Alex
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These ones are growing in a dish, but in a developing embryo or brain, each growth cone would be sniffing its way through a complex molecular terrain, often over long distances to find its correct synaptic partners.
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Hi Luc, thanks!
it is indeed Spy555-FastAct.
Cheers
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me too! They seem to be retraction fibers that are then fragmenting into these small vesicles. It has been described in some migrating cells/cancer cells before. We are now inestigating it in the context of axon growth and guidance!
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Yes, it is, I wrote it in the ALT text but somehow it I can't see it. SPY555-FastAct
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And sometimes accross the floor plate 🙂
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🤩
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Fascinating! Congrats!
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"I am a piece of debris!" haha
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It is showing off for sure! 🕺
Might be a piece of debris. Maybe a filopodium that still wants to party hard...
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you were already in ;)
cheers
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this overqualifies! you were already in the list. I just added your lab.
cheers
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Hi Monica,
May I please be added to the feed?
Thanks!
my ORCID: orcid.org/0000-0002-24...
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Mesmerizing, wasn't it? 🤩
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Thanks! I color-coded the stacks in Fiji using the fire LUT. You can find it under image>hyperstack. I found out that for data with multiple time points, you have to set time as z and z as time in re-order hyperstack function.
Cheers
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Thanks! A minority of the total pool of commissural axons is labeled. In fact, around 50% of the dI1 interneuron subpopulation is labeled. I used the Atoh1 enhancer and in ovo electroporation to target them.
Cheers
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done! :)
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Given the fact that embryonic neurons are very sensitive to the phototoxicity of these kind of dyes, it is amazing that one can even combine them now!👌
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thanks! no, that was actually the SPY650-FastAct 😉
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I'm ashamed to say how long I've been staring at this video. 😅
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Thanks!