bskytdp.bsky.social
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Proteomes vary in time and space/location/environment, so your suggestion is on the mark. Just to add, that's what makes genuinely deep proteomic analysis (at the proteoform level) so complex and critical, and why comparisons to the genome/genomics are not well considered.
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Hmmmm......doing science (notably proteomics) "in the dark" - it either explains so much about were we are or, gods forbid, it's foreshadowing...................
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......in space.
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Thought he is Borg?
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Reminds me of my mother's response decades ago when a door-to-door salesman shoved his foot in the door to keep it from being closed. She was a quiet, unassuming woman, but I doubt that salesman ever walked properly again after that door slammed shut. The same message needs to be sent.
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Seems quality overall in science is on a slippery slope, as is the management of universities.
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Definitely...& required a more appropriate response than that of most administrations. I think we're at a stage where funding alone won't fix problems because so much of it will already be spent (so to speak) before it even arrives. Reality must set in for real change to occur (e.g. Laurentian).
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The Joseph Goebbels approach to propaganda.....just another reason to be wary.
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Can it possibly be true?! There is genuinely deep, comprehensive proteomic analysis being done at the proteoform level!?! Only Integrated Top-Down Proteomics (iTDP) can address whole proteome analysis across the full MW & pI range of native proteomes.
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Not to mention seemingly ever-expanding senior and mid-level administrations that suck available funds from the actual mandate of universities. Fiscal responsibility has seemingly gone out the window. Sadly, it's actually an international issue, at least in the West.
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A former PhD student is currently postdoc'ing in a 'high throughput screening lab' at NIH, and this is one of their focuses (as in lysosomal disorders). So, definitely somewhat of a niche, but an interesting & important one. For years nobody thought endosomes or lysosomes even fused with the PM!
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BTW - even if Canadian universities found the money to pay the expected salaries of such US researchers, our research funding systems could not provide what they are used to in terms of running their research programs.
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Not disagreeing with anyone but, like personal finances, universities and their administrations must live within their means. One might rightly question if that has reasonably been the case or has a broadening of focus and initiatives meant that actual research & educational mandates have suffered?
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Any word search for 'lysosomes exocytosis' for example will yield an appropriate set of references to consider. Original work goes back to the mid-to-late 90's but has somewhat taken off as of approx 2010 or so.
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There is actually a whole literature on lysosomes as exocytotic organelles (i.e. fusing with the PM and thus extracellular release of contents).
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Lysosomes are actually also secretory organelles; contents released under selective stimuli.
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Just another example of how (hyper-)hype is hurting serious science....and it goes well beyond a couple of wolves.
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Wonder how many folks got a deal on the instrument in exchange for the 'advertising' titles (as though a published study using the instrument wasn't enough)? Unless the study was to specifically to assess the instrument, one has to wonder how necessary this is.
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Same 'problem' that has plagued proteomics since Day 1 (i.e. ~1975)...no labs using same protocols or workflow. Explains so much of the situation we find ourselves in, not just with respect to LIMS.
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Never too late... especially if you can bring new thinking to an area!
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That they want your money??
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Using Linux Mint for a year now on a recommendation from one of my sons.....very satisfied!
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Still rocking the original Samsung ultra-thin circa 2008-2009. No decals but the scars of much international travel, a memory upgrade, and a couple of new battery installs over the years. Invest in good equipment and it will serve you well.
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You sure that advice isn't from Padula?!?
My advice, don't go topless and oiled-up for the talk.....unless you've been doing a lot of weights lately? A folk instrument might help though.
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Fortunately, any strong advances in bottom-up technique actually best benefit the integrative top-down (iTDP) proteomic approach to genuinely deep proteome analysis. LC/MS/MS is the strong second stage of iTDP, which fully delivers by first using rigorous up-front resolution of intact proteoforms.
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So true. Strange how some researchers position themselves as critical, yet ignore reality for a simpler, 'faster' approach unlikely to give genuinely necessary data. If only investors in these initiatives had actually sought expert rather than simply 'mainstream' opinion before jumping in.
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.....but makes money.
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Somewhat the same issue with so-called 'hub' proteins or multi-function proteins which, more likely than not, actually represent several molecular species.
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I thought it was supposed to be 20,000? Granting agencies certainly seem to agree with you though......and big projects at certain universities, internationally.
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Hmmmm...is it a 'hang up' or recognition of what we really need to be deeply focused on in proteomics (and a genuine concern that inferring the presence of intact canonical sequences is how most work is still done)? We should chat sometime Mate.
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....and certainly not proteoforms.
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Sadly, stories similar to this abound at far too many universities, more widely than just in Australia.
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Cheers.
As you say, proving the absence is tough and I don't think anyone probably wants to search through all those studies to assess the quality of protein recovery/isolation nor the analytical processes employed.
Databases and atlases are what they are.
Hope all is well out your way.
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Thanks Ron. What I am concerned about is when one doesn't know exactly how the different prep's/isolations/etc were done, we can't even begin to suspect if/how any PTM might have been lost in process.
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Sure it's not the year of our Lady 2025? Regardless, it is strange at this point, for a topic such as memory mechanisms, that potential (likely?) gender differences were not accounted for. Maybe they needed to save enough money to be able to publish in Nature?
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......and then some!
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I've actually worked on lysosomes (both in the cell as well as isolated), and have done more than my share of various subcellular fractionations. So my interest is in how you actually got the proteins themselves for analysis, not the organelle.
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Ron - I was intrigued by this and your similar note on one of the PLA2's. But how were these isolated for analysis?
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Not to mention direct attempts at invasion - War of 1812 and even the Fenian Raids (1866 to 1871), the latter with the Americans 'quietly' pushing the local Irish to 'attack the British' across the boarder. Hmmm....prelude to CIA-style Latin American operations?
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Simply unfair to show something like this unless you have enough for everyone.
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It is clearly not worth pursuing this further since one of us has genuine international experience in senior admin and how things actually run and what defines efficiencies and responsibilities at that level. Perhaps Durham is running well, although you do seem to have mentioned large deficits......
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Yes, but you do help to get there by cutting expansive senior admin networks that include Assistant Associate VPs all of whom need several assistants who actually do the work. They have made themselves 'easy targets' by being fiscally irresponsible for years. Time for responsibility, not excuses.
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A good start at most public universities anywhere would be to drop ~50-70% of their overpaid, under-performing, and fiscally irresponsible senior administrators (most of whom are just waiting for a leg-up to their next overpaid position). What's happened to responsibility and dedication?
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Push a Viking Mom and pay the price. Lesson learned.
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Regrettably, such things are happening all over the world. Unis have largely become by-&-for administrators; everyone and everything else comes somewhere down the list. Most Unis have Economics Depts and/or Business Schools but senior admin never looks internally for guidance/solutions.
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Might be an emergency if Canada turns off the oil, electricity and uranium?! But then, of course, "They have nothing we need or want".
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'Updates their own training'.....and it can't be soon enough! Now they've renamed themselves 'People & Culture' but the individuals in charge clearly know nothing about either. Never seen such a pair of self-centred social climbers. Great staff though!
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So, grownups can do this without threatening espionage and/or invasion? Go figure.
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'Novelty' and 'innovation' may be two of the most abused words in science and business. That said, these cuts to funding will undoubtedly have long-term effects especially on ECR.